Enzyme Polymorphism in Sitophilus oryzae (Linnaeus, 1763) and Sitophilus zeamais (Motschulsky, 1855) (Coleoptera, Curculionidae) in southern Brazil

Danielle das Neves Bespalhok; Erasmo Renesto; Tiago Signorini

doi:10.4025/actascibiolsci.v37i2.25143

Enzyme Polymorphism in Sitophilus oryzae (Linnaeus, 1763) and Sitophilus zeamais (Motschulsky, 1855) (Coleoptera, Curculionidae) in southern Brazil

Danielle das Neves Bespalhok Universidade Estadual de Maringá
Erasmo Renesto Universidade Estadual de Maringá
Tiago Signorini Universidade Estadual de Maringá

DOI: https://doi.org/10.4025/actascibiolsci.v37i2.25143

Keywords: pest insect, stored grains, starch gel, electrophoresis, heterozygosity

Abstract

Beetles of the species Sitophilus oryzae and S. zeamais are pests of great economic importance since they attack not only rice and maize but also several other cereals. In fact, these beetles are one of the most visible threats to sustainable food production. Current study estimated the genetic variability of S. oryzae in two samples, one from the State of Paraná (PR), Brazil, and another from the state of Rio Grande do Sul (RS), Brazil, and a sample of S. zeamais from the State of Santa Catarina (SC), Brazil. Isozyme electrophoresis in starch gel technique was employed to analyze eight enzyme systems (AAT, ACP, GDH, GPI, IDH, MDH, PGM and ME). Average heterozygosity rates were 0.0091, 0.0100 and 0.0000 and expected heterozygosity rates were 0.0419, 0.0452 and 0.0000 respectively for the samples of PR, SC and RS samples. The percentage of polymorphic loci was 30% in the PR sample, 0% in the RS sample and 30% in the SC sample. Genetic identity rates were I=0.9983 between samples from PR and RS; I = 0.6892 between PR and SC, and I = 0.6925 between SC and RS. Nei´s (1978) genetic distance rates were 0.0017, 0.3722 and 0.3675. Samples presented low genetic variability.

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Author Biographies

Danielle das Neves Bespalhok, Universidade Estadual de Maringá

Graduada em Ciencias Biológicas pela Universidade Estadual de Maringá (2010). Mestrado no Programa de Pós-Graduação em Genética e Melhoramento da Universidade Estadual de Maringá (2012) e atualmente doutoranda no Programa de Pós-Graduação em Genética e Melhoramento da Universidade Estadual de Maringá.

Erasmo Renesto, Universidade Estadual de Maringá

Possui graduação em História Natural pela Universidade Estadual Paulista Júlio de Mesquita Filho (1970), mestrado em Ciências Biológicas (Genética) pela Universidade de São Paulo (1973) e doutorado em Ciências Biológicas (Genética) pela Universidade de São Paulo (1979). Atualmente é professor associado da Universidade Estadual de Maringá onde atua como orientador nos cursos de Ecologia de sistemas aquáticos continentais, Genética e Melhoramento e Biologia Comparada em pesquisas de variabilidade genética de populações naturais.

Published

2015-08-06

How to Cite

Bespalhok, D. das N., Renesto, E., & Signorini, T. (2015). Enzyme Polymorphism in Sitophilus oryzae (Linnaeus, 1763) and Sitophilus zeamais (Motschulsky, 1855) (Coleoptera, Curculionidae) in southern Brazil. Acta Scientiarum. Biological Sciences, 37(2), 205-211. https://doi.org/10.4025/actascibiolsci.v37i2.25143

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Issue

Vol 37 No 2 (2015)

Section

Genetics

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