The effect of spectral light quality on in vitro culture of sugarcane

Marina Medeiros de Araújo Silva; Arquimedes Liberal Barboza de Oliveira; Ronaldo Alves Oliveira-Filho; Terezinha Camara; Lilia Willadino; Artur Gouveia-Neto

doi:10.4025/actascibiolsci.v38i2.31109

The effect of spectral light quality on in vitro culture of sugarcane

Marina Medeiros de Araújo Silva Instituto Nacional do Semiárido
Arquimedes Liberal Barboza de Oliveira Universidade Federal Rural de Pernambuco
Ronaldo Alves Oliveira-Filho Universidade Federal Rural de Pernambuco
Terezinha Camara Universidade Federal Rural de Pernambuco
Lilia Willadino Universidade Federal Rural de Pernambuco
Artur Gouveia-Neto Universidade Federal Rural de Pernambuco

DOI: https://doi.org/10.4025/actascibiolsci.v38i2.31109

Keywords: in vitro propagation, light wavelength, Saccharum spp.

Abstract

The effects of different light quality treatments on in vitro growth and multiplication of sugarcane (RB867515) were investigated. The plantlets were cultivated on MS medium containing 1.3 μM 6-benzylaminopurine (BAP), and exposed to five light treatments: three combinations of blue/red LED (70:30, 50:50, 30:70), white-LED and fluorescent lamps, during 24 days. Among the LED light treatments, blue/red combination in 50:50 proportions proved to have the best results for stem length, fresh mass, leaf number and shoot multiplication. Higher content of photosynthetic pigments was also obtained with LEDs. Results suggested that the light quality emitted by LEDs was suitable for plant growth and development and it may be used as alternative and economic light source for micropropagation of sugarcane variety under analysis.

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Author Biographies

Marina Medeiros de Araújo Silva, Instituto Nacional do Semiárido

Doutora em Biologia Vegetal

Arquimedes Liberal Barboza de Oliveira, Universidade Federal Rural de Pernambuco

Agrônomo

Ronaldo Alves Oliveira-Filho, Universidade Federal Rural de Pernambuco

Agrônomo

Terezinha Camara, Universidade Federal Rural de Pernambuco

Professora do Departamento de Química

Lilia Willadino, Universidade Federal Rural de Pernambuco

Professora do Departamento de Biologia

Artur Gouveia-Neto, Universidade Federal Rural de Pernambuco

Professor do Departamento de Química

Published

2016-10-24

How to Cite

Silva, M. M. de A., Oliveira, A. L. B. de, Oliveira-Filho, R. A., Camara, T., Willadino, L., & Gouveia-Neto, A. (2016). The effect of spectral light quality on in vitro culture of sugarcane. Acta Scientiarum. Biological Sciences, 38(2), 157-161. https://doi.org/10.4025/actascibiolsci.v38i2.31109

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Issue

Vol 38 No 2 (2016)

Section

Biotechnology

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