In vitro inactivation of thrombin generation by polysulfated fractions isolated from the tropical coenocytic green seaweed Caulerpa racemosa (Caulerpaceae, Bryopsidales)

José Ariévilo Gurgel Rodrigues; Norma Maria Barros Benevides; Ana Maria Freire Tovar; Paulo Antônio de Souza Mourão

doi:10.4025/actascibiolsci.v39i3.32095

In vitro inactivation of thrombin generation by polysulfated fractions isolated from the tropical coenocytic green seaweed Caulerpa racemosa (Caulerpaceae, Bryopsidales)

José Ariévilo Gurgel Rodrigues Universidade Federal do Rio de Janeiro
Norma Maria Barros Benevides Universidade Federal do Ceará
Ana Maria Freire Tovar Universidade Federal do Rio de Janeiro
Paulo Antônio de Souza Mourão Universidade Federal do Rio de Janeiro

DOI: https://doi.org/10.4025/actascibiolsci.v39i3.32095

Keywords: Chlorophyta, complex glycans, sulfation pattern, clot.

Abstract

Pharmacological efficacy of Caulerpa racemosa (Chlorophyta) sulfated polysaccharidic (SPs) fractions (F I→III) on models of coagulation and inflammation has been demonstrated, but not their effects on thrombin generation (TG). This study examined fractions for composition and physical-chemical characteristics and in vitro inactivation of TG by F I and F II in 60-fold diluted human plasma using continuous method. Papain-extraction yield of 0.7% revealed F I→III by DEAE-cellulose chromatography, with differences among the relative proportions of sulfate (17.37-24.00%), total sugars (30.03-48.34%) and absence of proteins. Charge density patterns and molecular sizes > 100 kDa of the fractions were verified by both agarose/polyacrylamide analyses, respectively. These electrophoreses combined with toluidine blue/Stains-All also indicated nonSPs. Anticoagulant effects of 4.76 (F I), 12.00 (F II) and 2.32 (F II) IU mg^-1 by activated partial thromboplastin time test were recorded against heparin (193 IU mg^-1), without changes in prothrombin time. Diluted plasma treated with F I and F II reduced concentration-dependent and sulfation pattern TG by both intrinsic and extrinsic pathways, with 50% inactivation by intrinsic pathway of F II even at 4.1 µg. Heparin abolished TG at least 4-fold lower. Therefore, C. racemosa produces SPs with TG inhibition.

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Author Biography

José Ariévilo Gurgel Rodrigues, Universidade Federal do Rio de Janeiro

Graduado em Engenharia de Pesca pela Universidade Federal do Ceará (2004), mestrado em Recursos Pesqueiros e Engenharia de Pesca pela Universidade Federal do Ceará (2006) e doutorado em Biotecnologia pela Rede Nordeste de Biotecnologia - RENORBIO/UFC (2011). Tem experiência na área de Recursos Pesqueiros e Engenharia de Pesca e de Biotecnologia em Recursos Naturais, com ênfase em Taxonomia de Algas Marinhas, Fisiologia Comparada (taxonomia de grupos, filogenia e biogeografia) e Bioprospecçâo de Produtos Naturais Bioativos de Organismos Aquáticos. Atua nas seguintes áreas: Ficologia, Química de macromoléculas (polissacarídeos sulfatados) e suas propriedades químicas e implicações terapêuticas sobre coagulação, trombose, dor, inflamação e de avaliação toxicológica (aguda, subcrônica e crônica). Foi professor horista (prestador de serviços) do Instituto de Desenvolvimento, Educação e Cultura do Ceará (IDECC-UVA) e do Instituto Dom José de Educação e Cultura (IDJ-UVA). Atualmente, realiza Pós-Doutorado na Universidade Federal do Rio de Janeiro (CAPES-PNPD).

Published

2017-09-18

How to Cite

Rodrigues, J. A. G., Benevides, N. M. B., Tovar, A. M. F., & Mourão, P. A. de S. (2017). In vitro inactivation of thrombin generation by polysulfated fractions isolated from the tropical coenocytic green seaweed Caulerpa racemosa (Caulerpaceae, Bryopsidales). Acta Scientiarum. Biological Sciences, 39(3), 283-292. https://doi.org/10.4025/actascibiolsci.v39i3.32095

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Issue

Vol 39 No 3 (2017)

Section

Biotechnology

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