Prediction of mutations on structure primase of the archaeon Sulfolobus solfataricus

Eden Silva e Souza; Michely Correia Diniz

doi:10.4025/actascibiolsci.v39i4.34600

Prediction of mutations on structure primase of the archaeon Sulfolobus solfataricus

Eden Silva e Souza Universidade Federal do Vale do São Francisco http://orcid.org/0000-0002-0724-9576
Michely Correia Diniz Universidade Federal do Vale do São Francisco http://orcid.org/0000-0002-1960-4512

DOI: https://doi.org/10.4025/actascibiolsci.v39i4.34600

Keywords: in silico, PriL, 3D structure, DNA replication, primer, mutations.

Abstract

All living organisms need a DNA replication mechanism and it has been conserved in the three domains of life throughout evolutionary process. Primase is the enzyme responsible for synthesizing de novo RNA primers in DNA replication. Archaeo-Eukaryotic Primase (AEP) is the superfamily that typically forms a heterodimeric complex containing both a small catalytic subunit (PriS) and a large accessory noncatalytic subunit (PriL). Sulfolobus solfataricus is a model organism for research on the Genetics field. The aim of this work was to evaluate, via Bioinformatics tools, three mutations in the large subunit (PriL) of the archaeon Sulfolobus solfataricus. The aspartic acid residue in the positions (Asp) 62, (Asp) 235, (Asp) 241 have been substituted by glutamic acid (Glu). The highest positive free energy variation of the three substitutions analyzed occurred with the mutation at the (Asp) 241 site. The in silico analysis suggested that these mutations in PriL may destabilize its tridimensional structure interfering with replication mechanisms of Sulfolobus solfataricus. Moreover, it may also alter interactions with other molecules, making salt bridges, for instance.

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Author Biographies

Eden Silva e Souza, Universidade Federal do Vale do São Francisco

Graduando em Ciências Biológicas na Universidade Federal do Vale do São Francisco com interesse em Bioquímica, Bioinformática.

Michely Correia Diniz, Universidade Federal do Vale do São Francisco

possui graduação em Licenciatura e Bacharelado em Cïências Biológicas pela Universidade Estadual do Ceará (2004). Concluiu o Mestrado em Genética na Universidade Federal de Pernambuco com ênfase em Genética Molecular (2007). Concluiu o Doutorado em Biotecnologia (2011), com atividades focadas nos mecanismos intraflagelares de protozoários. É Professora Adjunta III da Fundação Universidade Federal do Vale do São Francisco - UNIVASF -, atuando nas áreas de Genética Molecular, Biologia Evolutiva, Biotecnologia e Bioinformática.

Published

2017-11-24

How to Cite

Souza, E. S. e, & Diniz, M. C. (2017). Prediction of mutations on structure primase of the archaeon Sulfolobus solfataricus. Acta Scientiarum. Biological Sciences, 39(4), 463-467. https://doi.org/10.4025/actascibiolsci.v39i4.34600

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Issue

Vol 39 No 4 (2017)

Section

Genetics

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