Multivariate optimization of Staphylococcus xylosus AD1 biomass production using sugarcane molasses plus yeast extract and soybean meal

Abstract

Staphylococcus xylosus is a microorganism that has important physiological and technological characteristics that make it suitable for use as a starter culture in fermented meat products. For the development of these products in the food industry, it is necessary to produce biomass by the multiplication of starter cultures using low-cost media. This study developed a culture medium based on sugarcane molasses (SCM) supplemented with yeast extract (YE) and soybean meal (SM) to produce S. xylosus AD1 biomass employing a Box Behnken multivariate optimization design, using the best concentrations of the constituents of the culture medium for S. xylosus AD1 growth. By combining the mathematical models by the desirability function, it was possible to establish the optimal condition for the maximum production of viable cells and biomass. The optimal experimental condition was found when the fermentative process medium was composed of 10% SCM, 2% YE and 4% SM. In addition, the results of all experiments, except for the medium formulated with only SCM, presented a better performance than the commercial medium Brain Heart Infusion for the growth of S. xylosus AD1. The culture medium with agro-industrial byproduct (SCM) supplemented with YE and SM is an excellent alternative for producing S. xylosus AD1 biomass.