Utilização da serologia na identificação de Ascia monuste orseis (Latreille, 1819) (Lepidoptera: Pieridae) - DOI: 10.4025/actascibiolsci.v31i2.515

Fabiano de Mello Costa; Ana Paula da Silva Loffredo; Helena Caroline Onody; Carlos Roberto Sousa-Silva

doi:10.4025/actascibiolsci.v31i2.515

Using the serological technique to identify Ascia monuste orseis (Latreille, 1819) (Lepidoptera: Pieridae) - DOI: 10.4025/actascibiolsci.v31i2.515

Fabiano de Mello Costa UFSCAR
Ana Paula da Silva Loffredo UFSCAR
Helena Caroline Onody UFSCAR
Carlos Roberto Sousa-Silva UFSCAR

DOI: https://doi.org/10.4025/actascibiolsci.v31i2.515

Keywords: Insecta, biological control, antiserum, predation

Abstract

This study aimed to obtain specific antiserum for Ascia monuste orseis (Latreille, 1819), with the aim of identifying the predators associated with this insect. The preparation of specific antiserum was performed by immunizing a rabbit via a lymph nodule injection containing antigen obtained from the maceration of 4^th and 5^th instars of A. monuste orseis caterpillars. Serological homologous tests performed using double diffusion in agar gel were positive 21 days after the first antigen inoculation. Eggs, 1^th, 2^th and 3^th instar caterpillars, pupae and adults of A. monuste orseis also reacted positively to the obtained antiserum.

Downloads

Download data is not yet available.

Author Biography

Helena Caroline Onody, UFSCAR

Tem experiência na área de Zoologia, com ênfase em Entomologia, atuando principalmente nos seguintes temas: taxonomia e ecologia de Hymenoptera Parasitóides em áreas naturais e agroecossistemas, estudo das interações parasitóide-hospedeiro e avaliação dos efeitos de extratos vegetais em pragas agrícolas Currículo Lattes

PDF (Português (Brasil))

Published

2009-05-14

How to Cite

Costa, F. de M., Loffredo, A. P. da S., Onody, H. C., & Sousa-Silva, C. R. (2009). Using the serological technique to identify Ascia monuste orseis (Latreille, 1819) (Lepidoptera: Pieridae) - DOI: 10.4025/actascibiolsci.v31i2.515. Acta Scientiarum. Biological Sciences, 31(2), 149-151. https://doi.org/10.4025/actascibiolsci.v31i2.515

Download Citation

Issue

Vol 31 No 2 (2009)

Section

Ecology and Limnology

DECLARATION OF ORIGINALITY AND COPYRIGHTS

I Declare that current article is original and has not been submitted for publication, in part or in whole, to any other national or international journal.

The copyrights belong exclusively to the authors. Published content is licensed under Creative Commons Attribution 4.0 (CC BY 4.0) guidelines, which allows sharing (copy and distribution of the material in any medium or format) and adaptation (remix, transform, and build upon the material) for any purpose, even commercially, under the terms of attribution.

Read this link for further information on how to use CC BY 4.0 properly.