Effect of Leishmania proteins in mice: Investigation of Leishmania amazonensis virulence molecules for therapy and prophylaxis of leishmaniasis

Cintia Figueiredo de  Araújo; Candace Machado  Andrade; Mariana Ivo  Khouri; André Cronemberger  Andrade; Luciana Souza de  Aragão-França; Viviane Costa Junqueira  Rocha; Virgínia Maria Góes da  Silva; Lain Carlos Pontes de  Carvalho

doi:10.4025/actascibiolsci.v47i1.70466

Cintia Figueiredo de Araújo Fundação Oswaldo Cruz - Fiocruz https://orcid.org/0000-0002-5579-0981
Candace Machado Andrade Centro Universitário Jorge Amado https://orcid.org/0000-0003-4467-7016
Mariana Ivo Khouri Fundação Oswaldo Cruz - Fiocruz https://orcid.org/0000-0003-4371-4241
André Cronemberger Andrade Paris Cité University https://orcid.org/0000-0002-1790-081X
Luciana Souza de Aragão-França Universidade Federal da Bahia https://orcid.org/0000-0002-3678-0411
Viviane Costa Junqueira Rocha Fundação Oswaldo Cruz - Fiocruz https://orcid.org/0009-0002-4968-1305
Virgínia Maria Góes da Silva Universidade Estadual do Suldoeste da Bahia https://orcid.org/0000-0003-4999-0597
Lain Carlos Pontes de Carvalho Fundação Oswaldo Cruz - Fiocruz https://orcid.org/0000-0003-0847-7906

DOI: https://doi.org/10.4025/actascibiolsci.v47i1.70466

Keywords: Leishmania amazonensis; Leishmania braziliensis; Antigen; Infection; Leishmaniasis.

Abstract

It has been reported that a single injection of Leishmania amazonensis amastigote extract (LaE) in BALB/c mice exacerbates the infection of these animals with Leishmania braziliensis. This study aimed to investigate proteins of LaE that confers susceptibility to L. braziliensis infection. Methods BALB/c mice were injected with saline, LaE, isolate fractions of the LaE (68, 45, 36, and 28 kDa), or L. infantum recombinant protein Lci1 (Hsp70). After a week, the animals were infected with L. braziliensis, and the lesion’s size was monitored weekly. Six weeks later, the parasite burden in the footpads and the activities of IgG1 and IgG2a anti-LaE antibodies were determined. Additionally, the 68 and 28 kDa fractions were supplemented with serine and cysteine protease inhibitors and evaluated in the same described experimental model. The intradermal injection of BALB/c mice with 68, 36, and 28 kDa fractions of LaE, and the recombinant protein Lci1 conferred significant susceptibility to L. braziliensis infection, but not the 45 kDa fraction. This potentiating effect was associated with the increase in increment of the lesion size, parasite load, and IgG1 anti-LaE antibodies. Furthermore, the biological activity of the 68 and 28 kDa fractions was inhibited by the pretreatment of the extract with serine protease inhibitors. Our study found the biological activity of the 68, 36, and 28 kDa fractions, as well as the recombinant protein Lci1, exacerbating the infection in our experimental model.

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