Isolation of fungi potentially producing enzymes of agroindustrial interest: A qualitative analysis of the standardization of the LED light Method
Abstract
The biotechnology industry plays a crucial role in the advancement of agronomy, contributing increasingly to each stage of its development. Microbial bioprospecting is a highly diverse field with significant agroindustrial potential, as nature provides a vast range of microorganisms suitable for such applications — including lipase-producing strains. As part of an initiative to establish a fungal collection at the Universidade do Estado de Minas Gerais (UEMG) – Passos Unit, 22 fungal isolates were obtained from various natural sources, 17 of which demonstrated lipase production. Fungal isolates with the highest lipolytic activity were derived from wet soil in calf paddocks, oral residues from calves post-nursing, and treated sewage sludge. This study introduces a standardized method using LED light for detecting lipolytic activity — a technique that to the best of our knowledge has not previously been applied to microorganisms. LED light presents a safer alternative to UV light, which can compromise sample integrity and pose health risks to users. This novel approach highlights the diversity of lipase-producing fungi from distinct environmental sources. The study aims to evaluate and qualitatively assess fungal enzymatic activity through this innovative methodology, facilitating the selection of strains with high enzymatic potential for agroindustrial applications.
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References
Adrio, J. L., & Demain, A. L. (2014). Microbial enzymes: Tools for biotechnological processes. Biomolecules, 4(1), 117-139. https://doi.org/10.3390/biom4010117
Almeida, J. E. M. (2020). Biofábricas para produção de micopesticidas no Brasil: Oportunidades de negócio e inovações. Brazilian Journal of Animal and Environmental Research, 3(3), 2444-2557. https://doi.org/10.34188/bjaerv3n3-162
Ameri, A., Shakibaie, M., Amirpour-Rostami, S., Ameri, A., Adeli-Sardou, M., Khazaeli, P., & Forootanfar, H. (2015). Partial purification and characterization of a thermoalkalophilic lipase originated from Bacillus atrophaeus FSHM2 and its application for ester synthesis. Biotechnology, 14(4), 154-164. https://doi.org/10.3923/biotech.2015.154.164
Balogh, T. S., Velasco, M. V. R., Pedriali, C. A., Kaneko, T. M., & Baby, A. R. (2011). Proteção à radiação ultravioleta: Recursos disponíveis na atualidade em fotoproteção. Anais Brasileiros de Dermatologia, 86, 732-742. https://doi.org/10.1590/S0365-05962011000400016
Batista, B. N., & Soares, C. E. V. F. (2023). Microrganismos produtores de lipases para uso na produção de Biodisel. Revista OWL (OWL Journal) – Revista Interdisciplinar de Ensino e Educação, 1(2), 413-427. https://doi.org/10.5281/zenodo.8361746
Bessa, D. H. R. F., Flumignan, D. L., Souza, A. O., Gonçalves, M. C. M., & de Souza Castro, C. F. (2022). Obtenção de caldo bruto enzimático a partir de fungos lipolíticos para a produção de ésteres metílicos. Revista em Agronegócio e Meio Ambiente, 15(3), 1-13. https://doi.org/10.17765/2176-9168.2022v15n3e9279
Boonmahome, P., & Mongkolthanaruk, W. (2013). Lipase-producing bacterium and its enzyme characterization. J Life Sci Technol, 1(4), 196-200. https://doi.org/10.12720/jolst.1.4.196-200
Fontes, A. L., Pimentel, L. L., Soares, A. M. S., do Rosário Domingues, M., Rodríguez-Alcalá, L. M., & Gomes, A. M. (2023). Study of the viability of using lipase-hydrolyzed commercial vegetable oils to produce microbially conjugated linolenic acid-enriched milk. Food Chemistry, 413, 135665. https://doi.org/10.1016/j.foodchem.2023.135665
Gilham, D., & Lehner, R. (2005). Techniques to measure lipase and esterase activity in vitro. Methods, 36(2), 139-147. https://doi.org/10.1016/j.ymeth.2004.11.003
Gullan, P. J., & Cranston, P. S. (2017). Insetos: Fundamentos da entomologia (5a ed.). Editorial ROCA.
Gupta, R., Gupta, N., & Rathi, P. J. A. M. (2004). Bacterial lipases: An overview of production, purification and biochemical properties. Applied Microbiology and Biotechnology, 64, 763-781. https://doi.org/10.1007/s00253-004-1568-8
Kouker, G., & Jaeger, K. E. (1987). Specific and sensitive plate assay for bacterial lipases. Applied and Environmental Microbiology, 53(1), 211-213. https://doi.org/10.1128/aem.53.1.211-213.1987
Lock, L. L., Corbellini, V. A., & Silva, P. V. D. (2007). Lipases produced by yeasts: Powerful biocatalysts for industrial purposes. Tecno-lógica, 11(1-2), 18-25.
Maciel, V. F. A., Pacheco, T. F., & Gonçalves, S. B. (2010). Padronização do uso de corante rodamina B para avaliação de atividade lipolítica em estirpes fúngicas.
Melani, N. B., Tambourgi, E. B., & Silveira, E. (2020). Lipases: From production to applications. Separation & Purification Reviews, 49(2), 143-158. https://doi.org/10.1080/15422119.2018.1564328
Mora, M. A. E., Castilho, A. M. C., & Fraga, M. E. (2016). Fungos entomopatogênicos: Enzimas, toxinas e fatores que afetam a diversidade. Revista Brasileira de Produtos Agroindustriais, 18, 335-349.
Oliveira Carvalho, P., Contesini, F. J., Bizaco, R., & Alves Macedo, G. (2005). Kinetic properties and enantioselectivity of the lipases produced by four Aspergillus species. Food Biotechnology, 19(3), 183-192. https://doi.org/10.1080/08905430500316342 (Referência não foi citada no texto)
Oliveira, T. F. D., Hidálgo, M. R., & Soares Júnior, M. S. (2014). Production of lipase extrated from aqueous waste: Enzymatic activity kinetics. Ciência e Agrotecnologia, 38(6), 562-572. https://doi.org/10.1590/S1413-70542014000600005
Peil, G. H., KuSS, A. V., Rave, A. F., Villarreal, J. P., Hernandes, Y. M., & Nascente, P. S. (2016). Bioprospecting of lipolytic microorganisms obtained from industrial effluents. Anais da Academia Brasileira de Ciências, 88, 1769-1779. https://doi.org/10.1590/0001-3765201620150550
Rabbani, M., Bagherinejad, M. R., Sadeghi, H. M., Shariat, Z. S., Etemadifar, Z., Moazen, F., & Zaghian, S. (2013). Isolation and characterization of novel thermophilic lipase-secreting bacteria. Brazilian Journal of Microbiology, 44, 1113-1119. https://doi.org/10.1590/S1517-83822013000400013
Rodrigues, C., Cassini, S. T., Antunes, P. W., Keller, R. P., & Gonçalves, R. F. (2016). Isolation and selection of lipase-producing fungi based on lipase activity and hydrolytic potential on soybean oil and grease trap scum. Engenharia Sanitária e Ambiental, 21, 507-518. https://doi.org/10.1590/S1413-41522016141401
Sharma, R., Chisti, Y., & Banerjee, U. C. (2001). Production, purification, characterization, and applications of lipases. Biotechnology Advances, 19(8), 627-662. https://doi.org/10.1016/S0734-9750(01)00086-6
Singh, M., Chandraveer, T. A., & Tripathi, A. (2017). Isolation and screening of lipase producing microorganisms from natural sources. Indian Journal of Ecology, 44(1), 19-23.
Soares, I. A., Flores, A. C., Mendonça, M. M., Barcelos, R. P., & Baroni, S. (2020). Fungos na biorremediação de áreas degradadas. Arquivos do Instituto Biológico, 78, 341-350. https://doi.org/10.1590/1808-1657v78p3412011
Song, H. T., Jiang, Z. B., & Ma, L. X. (2006). Expression and purification of two lipases from Yarrowia lipolytica AS 2.1216. Protein Expression and Purification, 47(2), 393-397. https://doi.org/10.1016/j.pep.2006.02.007
Veerapagu, M., Narayanan, A. S., Ponmurugan, K., & Jeya, K. R. (2013). Screening selection identification production and optimization of bacterial lipase from oil spilled soil. Asian Journal of Pharmaceutical and Clinical Research, 6(3), 62-67.
Verma, N., Thakur, S., & Bhatt, A. K. (2012). Microbial lipases: Industrial applications and properties (a review). International Research Journal of Biological Sciences, 1(8), 88-92.
Winayanuwattikun, P., Kaewpiboon, C., Piriyakananon, K., Chulalaksananukul, W., Yongvanich, T., & Svasti, J. (2011). Immobilized lipase from potential lipolytic microbes for catalyzing biodiesel production using palm oil as feedstock. African Journal of Biotechnology, 10(9), 1666-1673. https://doi.org/10.5897/AJB10.1802
Zuridah, H., Norazwin, N., Siti Aisyah, M., Fakhruzzaman, M. N. A., & Zeenathul, N. A. (2011). Identification of lipase producing thermophilic bacteria from Malaysian hot springs. African Journal of Microbiology Research, 5(21), 3569-3573. https://doi.org/10.5897/AJMR11.777
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