Isolamento e caracterização parcial de sequências homólogas a genes ribossomais (rDNA) em Blastocladiella emersonii - DOI: 10.4025/actascibiolsci.v25i2.2037

Luciano Seraphim Gasques; Maria Aparecida Fernandez; Luiz Carlos Correa

doi:10.4025/actascibiolsci.v25i2.2037

Isolation and partial characterization of homologous sequences of ribosomal genes (rDNA) in Blastocladiella emersonii

Luciano Seraphim Gasques Universidade Paranaense
Maria Aparecida Fernandez UEM
Luiz Carlos Correa UEM

DOI: https://doi.org/10.4025/actascibiolsci.v25i2.2037

Abstract

The definition and the characterization of replication origins regions in higher eukaryotes are still controversial. The initiation of the replication is site-specific in some systems but seems to occur in large regions in others. Because of its in tandem organization, reducing the area to the restricted space that codifies an unit of transcription, rDNA regions are attractive models to study replication origins. In this work we isolated and started to characterize a clone that contains a ribosomal sequence from the aquatic fungus B. emersonii, Be97M20. Southern blots showed several sites for the restrition enzymes Eco RI, HindIII and SalI. A northern blot of total RNA, hybridized against a probe made from Be97M20, confirmed its homology with the ribosomal 18S gene. The detailed characterization, including complete restriction map, subcloning, sequence and analysis on bidimensional gels will provide important information about the structure and dynamics of this region

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Author Biography

Luiz Carlos Correa, UEM

Possui graduação em Ciências Biológicas Modalidade Médica pela Universidade de São Paulo (1979), Mestrado em Bioquímica pela Universidade de São Paulo (1983), Doutorado em Bioquímica pela Universidade de São Paulo (1990) e Pós-doutorado na Université Paris XI, França (1994). Atualmente é professor adjunto da Universidade Estadual de Maringá. Tem experiência na área de Bioquímica, com ênfase em Bioquímica dos Microorganismos, atuando principalmente nos seguintes temas: Blastocladiella emersonii, esporulação, diferenciação celular, fosfatase ácida e Saccharomyces cerevisiae. Experiência admnistrativa: Diretor Geral da Pró-reitoria de Pesquisa e Pós-graduação da UEM; Chefe do Departamento de Bioquímica da UEM; Diretor de Assuntos Acadêmicos da UEM Currículo Lattes

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Published

2008-04-17

How to Cite

Gasques, L. S., Fernandez, M. A., & Correa, L. C. (2008). Isolation and partial characterization of homologous sequences of ribosomal genes (rDNA) in Blastocladiella emersonii. Acta Scientiarum. Biological Sciences, 25(2), 445-451. https://doi.org/10.4025/actascibiolsci.v25i2.2037

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Issue

Vol 25 No 2 (2003)

Section

Biology Sciences

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