Diversidade genética de populações naturais do Trypanosoma cruzi no Paraná, Sul do Brasil - DOI: 10.4025/actascihealthsci.v29i1.101

Leila Zalloum; Mônica Lúcia Gomes; Andréia Tieko Kinoshita; Max Jean de Ornelas Toledo; Alberto José Prioli; Silvana Marques Araújo

doi:10.4025/actascihealthsci.v29i1.101

**Genetic diversity of Trypanosoma cruzi natural populations in Paraná state, southern Brazil** - DOI: 10.4025/actascihealthsci.v29i1.101

Authors

Leila Zalloum UEM Author
Mônica Lúcia Gomes UEM Author
Andréia Tieko Kinoshita UEM Author
Max Jean de Ornelas Toledo UEM Author
Alberto José Prioli UEM Author
Silvana Marques Araújo UEM Author

DOI:

https://doi.org/10.4025/actascihealthsci.v29i1.101

Keywords:

Trypanosoma cruzi, grupos genéticos, Norte do Paraná, RAPD, SSR-PCR

Abstract

The genetic characteristics of twelve Trypanosoma cruzi strains isolated from chronic chagasic patients, triatomines and sylvatic reservoirs from Paraná state, Southern Brazil, were studied using the randomly amplified polymorphic DNA (RAPD) and simple sequence repeat-anchored polymerase chain reaction amplification (SSR-PCR) techniques. Sylvio and Esmeraldo stocks were used as reference for T. cruzi I and II, respectively. The DNA was amplified using three different primers for RAPD and ^(CA)8RY primer for SSRPCR. Gel analyses were scored by eye for a numerical taxonomy analysis based on the proportion of shared bands and by employing computer programs. The phenograms showed the existence of two distinct genetic groups: one containing the strains from chronic chagasic patients grouped with Esmeraldo and the other with the strains from triatomines and sylvatic reservoirs grouped with Sylvio. The existence of two phylogenetic groups of T. cruzi in Paraná state, Southern Brazil, is described for the first time.

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Author Biography

Silvana Marques Araújo, UEM

Possui graduação em Farmácia pela Universidade Federal de Minas Gerais (1979), mestrado em Parasitologia pela Universidade Federal de Minas Gerais (1985) e doutorado em Parasitologia pela Universidade Federal de Minas Gerais (1994). Atualmente é professor associado C da Universidade Estadual de Maringá. Tem experiência na área de Parasitologia, com ênfase em Protozoologia e Helmintologia, atuando principalmente nos seguintes temas: biologia molecular de Trypanosoma cruzi, melhoria do atendimento ao portador de sorologia positiva para doença de Chagas Currículo Lattes

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Published

2007-12-18

Issue

Vol. 29 No. 1 (2007)

Section

Clinical Analysis

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How to Cite

Zalloum, L., Gomes, M. L., Kinoshita, A. T., Toledo, M. J. de O., Prioli, A. J., & Araújo, S. M. (2007). Genetic diversity of Trypanosoma cruzi natural populations in Paraná state, southern Brazil - DOI: 10.4025/actascihealthsci.v29i1.101. Acta Scientiarum. Health Sciences, 29(1), 25-31. https://doi.org/10.4025/actascihealthsci.v29i1.101