Metodologia de seleção de cepas para produção etodologia da ciclodextrina glicosiltransferase e para purificação da enzima - DOI: 10.4025/actascihealthsci.v29i1.133

Glauciane de Lara Costa; Rúbia Pazzetto; Franciele Brol; Graciette Matioli

doi:10.4025/actascihealthsci.v29i1.133

Strains selection methodology for cyclodextrin glycosyltransferase production and enzyme purification - DOI: 10.4025/actascihealthsci.v29i1.133

Authors

Glauciane de Lara Costa UEM Author
Rúbia Pazzetto UEM Author
Franciele Brol UEM Author
Graciette Matioli UEM Author

DOI:

https://doi.org/10.4025/actascihealthsci.v29i1.133

Keywords:

ciclodextrina, glicosiltransferase, CGTase

Abstract

Cyclodextrins (CDs) are maltooligosaccharides produced from starch by cyclodextrin glycosyltransferase (CGTase) enzyme. This research aimed at establishing method for strains selection for CGTase production and enzyme purification. The microorganisms were selected from 53 analyses of starch cultures soils on plates containing specific culture medium for strains selection that produce CGTase. The enzymes were obtained by culturing these microorganisms in liquid medium. The enzyme activity was determined with photospectrometric methods and precipitation with trichloroethylene. The strain isolated from oat soil was the one that showed the highest activity [0.1864 µmol of ß-CD (min mL)-1]. This strain was used for enzyme production in laboratory scale and purification by biospecific affinity chromatography. The strain selected in this research opens new perspectives for enzymes production and CDs in industrial scale.

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Author Biography

Graciette Matioli, UEM

Possui graduação em Farmácia Bioquímica pela Universidade Estadual de Maringá (1985), mestrado em Ciências de Alimentos pela Universidade Estadual de Londrina (1991), doutorado em Ciências (Bioquímica) pela Universidade Federal do Paraná (1997) e pós-doutorado em Ciências de Alimentos pela Universidade Estadual de Campinas (2001). Atualmente é Professora Associada nível A - TIDE da Universidade Estadual de Maringá. Tem experiência na área de Farmácia Industrial, com ênfase em Biotecnologia e Ciências de Alimentos, atuando principalmente nos seguintes temas: ciclodextrina, ciclodextrina glicosiltransferase, enzimas, ciÃ«ncias de alimentos, complexos de inclusao, alimentos funcionais.

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Published

2007-12-18

Issue

Vol. 29 No. 1 (2007)

Section

Pharmacy

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How to Cite

Costa, G. de L., Pazzetto, R., Brol, F., & Matioli, G. (2007). Strains selection methodology for cyclodextrin glycosyltransferase production and enzyme purification - DOI: 10.4025/actascihealthsci.v29i1.133. Acta Scientiarum. Health Sciences, 29(1), 45-50. https://doi.org/10.4025/actascihealthsci.v29i1.133