Produção e purificação de anticorpos policlonais para Salmonella Enteritidis (Enterobacteriaceae)

Iliana Alcocer; Eiko Itano; Mario Augusto Ono; Tereza Cristina R.M. de Oliveira

doi:10.4025/actascihealthsci.v24i0.2483

Production and purification of polyclonal antibodies for Salmonella Enteritidis (Enterobacteriaceae)

Authors

Iliana Alcocer UEL Author
Eiko Itano UEL Author
Mario Augusto Ono UEL Author
Tereza Cristina R.M. de Oliveira UEL Author

DOI:

https://doi.org/10.4025/actascihealthsci.v24i0.2483

Keywords:

Salmonella Enteritidis, ELISA, anticorpo policlonal, meios de cultura

Abstract

The purpose of this study was to produce and to purify specific polyclonal antibodies for Salmonella Enteritidis (Enterobacteriaceae). The anti-serum was raised in rabbits using purified flagelin. Anti-serum titer and specificity were determined by an immunoassay - ELISA and its purification was performed by sepharose protein A affinity chromatography. The bacteria suspensions were cultivated in five different media (brain heart infusion - BHI, tripticase soy broth, nutrient broth - NB, peptone water). Results have showed that anti-serum titers varied depending on which media type was used and BHI and NB media yielded the most significant results. The anti-serum produced was specific for Salmonella Enteritidis. Its cross-reactivity with Salmonella Thyphimurium, Salmonella Infantis and Salmonella Newport were 16.0, 11.9 and 6.4% respectively and lower percentages than those were observed in other Enterobacteriaceae species tested. Results indicate that these polyclonal antibodies may be used in standardization of immunological assays for Salmonella Enteritidis detection

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Published

2008-04-24

Issue

Vol. 24 (2002)

Section

Health Sciences

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How to Cite

Production and purification of polyclonal antibodies for Salmonella Enteritidis (Enterobacteriaceae). (2008). Acta Scientiarum. Health Sciences, 24, 671-679. https://doi.org/10.4025/actascihealthsci.v24i0.2483