<b>Validation of analytical methods for quantifying captopril in tablets – a comparison of methodologies for a quality control program</b> - DOI: 10.4025/actascihealthsci.v26i2.1590

Abstract

Nowadays, when all approaches lead to the search for total quality, a thorough knowledge of every stage of a production process is vital. Validation is an appropriate tool to guarantee the reliabilities of: productive process installation, equipment and also analytical methodology. The aim of the present study was to analyze the main aspects of analytical methods validation for quantifying captopril. Validation and comparison of the following methods were carried out: titrimetric for oxide-reduction, spectrophotometry for Folin-Ciocalteau and high-efficiency liquid chromatography - HPLC. The attributes of accuracy, precision, linearity, specificity and robustness were studied for each methodology. The results show that the chromatographic method was the most suitable for Captopril 25 mg tablets evaluation, while the spectrophotometric method and the titrimetric method showed values that satisfied the acceptance criteria, although with higher variability and lower sensitivity.