<b>Intracytoplasmic sperm injection procedure for infertility treatment in couples</b> - DOI: 10.4025/actascihealthsci.v25i1.2242

Abstract

The ICSI procedure was used in a group of 68 patients (mean age 34,7 years). Ovarian stimulation, preparation of spermatozoa, preparation of the oocytes, direct injection of a single spermatozoon into the ooplasm and embryos replaced in uterus were done. The results of the present work demonstrate that 56% of diagnostic infertility categories were due to female factors. The highest frequency of male and female factors which caused infertility were asthenozoospermia (63%) and tubal factors (32,7%), respectively. From a total of 695 oocyte aspirated, 453 (67,1%) normal zygotes with two pronuclei (2 PN zygotes) were produced; 4 (0,55%), 32 (4,42%) and 2 (0,27%) resulted in 1 PN, 3PN and 4 PN zygotes respectively. The pregnancy rates by cycle and by transference were 25% and 28,9% respectively. Our data suggest that the ICSI procedure has been the method of choice in the treatment of infertility due to different etiology and the results showed that the successful fertilization rates has increased the chances of pregnancy.