<b>Study of drugs susceptibility and molecular samples evaluation of <em>Enterococcus</em> spp isolated in hospitalized patients</b> - DOI: 10.4025/actascihealthsci.v25i1.2249

  • João Bedendo UEM
  • Vera Lucia Dias Siqueira UEM
  • Celso Luis Cardoso UEM
  • Sueli Donizete Borelli UEM

Abstract

The aim of this study was to investigate the prevalency of Enterococcus spp, isolated from different clinical materials. The strains were evaluated for drugs susceptibility, typing by JB1-PCR and screened to the presence of Van A and Van B gene. From the 58 strains, 98.3% were classified as Enterococcus faecalis. All strains were sensible to nitrofurantoin and vancomycin, 96.6% were sensible to ampicilin, 32.8% were resistant to streptomycin, 10.3% were resistant to gentamicin and ciprofloxacin and 6.9% were resistant to norfloxacin. Using CIM test to check vancomycin susceptibility was observed bacterial growth until 4μg/ml. The presence of Van A e Van B gene was not detected. The amplification by JB1-PCR provided band patterns with up to 7 fragments and the samples were classified into 6 distint groups. The band patterns did not point out prevalence of any clone disseminated among strains. It was not possible to correlate genotype with antimicrobial resistance.

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Author Biography

João Bedendo, UEM
Possui graduação em Enfermagem pela Universidade Estadual de Londrina (1978), mestrado em Enfermagem pela Universidade de São Paulo (1988) e doutorado em Doenças Infecciosas e Parasitárias pela Universidade Federal de São Paulo (1998). Atualmente é prof associado da Universidade Estadual de Maringá. Tem experiência na área de Enfermagem, com ênfase em Enfermagem Médico-Cirúrgica, atuando principalmente nos seguintes temas: pcr, hla, s.aureus, infecção hospitalar, método sorológico e tipagem Currículo Lattes
Published
2008-04-17
How to Cite
Bedendo, J., Siqueira, V. L. D., Cardoso, C. L., & Borelli, S. D. (2008). <b>Study of drugs susceptibility and molecular samples evaluation of <em>Enterococcus</em> spp isolated in hospitalized patients</b&gt; - DOI: 10.4025/actascihealthsci.v25i1.2249. Acta Scientiarum. Health Sciences, 25(1), 35-40. https://doi.org/10.4025/actascihealthsci.v25i1.2249
Section
Health Sciences

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0.3
2019CiteScore
 
 
8th percentile
Powered by  Scopus