Application of the method of PCR-RFLP to the knowledge of HPV types in cervical infections from patients assisted in Lepac, State University of Maringá (UEM)

Abstract

The method of Polymerase Chain Reaction associated with Restriction Fragment Length Polymorphism (PCR-RFLP) was applied in 20 cervical clinical samples from patients with cytologic abnormalities, assisted in LEPAC-UEM. The DNA and the type of HPV were detected and determined in all of the analyzed samples. The great majority of the cervical samples showed HPV types, with high potential for neoplasia development. The method of PCR-RFLP proved great discrimination power, because it was able to detect the DNA and to determine the potential of neoplasia development for up to four different HPV types present in the same cervical sample. The results obtained demonstrated that this method can be used routinely, with high sensibility and reprodutibility, being a choice method in primary screen of patients with risk of developing cervical cancer, contributing with information that help a possible control of this disease.