<b>Production of tumoral necrosis factor-alpha and hydrogen peroxide during <em>Trypanosoma cruzi</em> infection in mice submitted to exercise</b> - DOI: 10.4025/actascihealthsci.v32i1.6757

Abstract

This study aimed to determine the levels of seric tumor necrosis factor-alpha (TNF-alpha) and production of hydrogen peroxide (H₂O₂) by peritoneal macrophages in mice experimentally infected with T. cruzi and submitted to pre-infection exercise training and to post-infection acute exercise. Female 30-day-old BALB/c mice were inoculated with 1,400 blood trypomastigotes of Y-strain T. cruzi. Exercise programs consisted in moderate-intensity activity and were carried out in a treadmill. The measurements were performed with material collected at the 13^th day after infection Serum TNF-alpha was evaluated using capture ELISA. H₂O₂ production was expressed by coloration produced after incubation of peritoneal macrophages and the measurement was performed using an ELISA reader. There were no statistically significant differences in TNF-alpha levels and H₂O₂ production between the trained and non-trained infected groups. Thus, the physical training performed before infection and physical exercise performed after the infection were not able to change the levels of TNF-alpha and production of H₂O₂ in the infection by T. cruzi.