PROTOCOLO RÁPIDO, FÁCIL E DE ALTO RENDIMENTO DE ISOLAMENTO DE DNA GENÔMICO TOTAL DE <em>Colletotrichum gloeosporioides</em> E <em>Fusarium oxysporum</em>

  • Eiko Eurya Kuramae-Izioka UNESP

Abstract

A rapid, easy and simple protocol for total genomic DNA isolation was developted for Colletotrichum gloeosporioides and Fusarium oxysporum. The total genomic DNA yield was very high and suitable to RAPD (random amplified polymorphic DNA), and restriction endonuclease reactions. DNA obtained by this protocol was not contaminated by protein or carbohydrate, and proteases, cesium chloride or phenol were also not necessary.

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Published
2008-07-28
Section
Agronomy