COMPARISON OF SUBSTRATES USED TO DETECT TOXINS PRODUCED BY <em>Aspergillus flavus</em> IN SOYBEAN
Abstract
Different substrates were evaluated regarding their capacity for detecting toxins produced by toxic species of Aspergillus flavus. The easy preparation of substrate, its quick reading, contaminations and the possibility of large scale production were also considered. Agar-coconut, agar-coconut with cellophane, agar-water and filter paper were the substrates analyzed. The macroorganism chosen was the toxigenic Aspergillus flavus, isolated from soybean seeds and transferred to Czapeck-agar. The laminae containing fungi were transferred to each substrate. The 24, 48, 72 and 96-hours readings after installation determined the fluorescent intensity produced in long wave ( 365nm) U.V. light while the radial mycelial growth was determined by the colony diameter. The best substrate was agar-coconut which showed medium and high fluorescence intensity after 48-hours incubation, and higher growth in relation to the remaining substrates, mainly the ones with cellophane. The agar-water and filter paper with cellophane substrates were the least efficient obtaining only low intensity fluorescence in approximately 50% of the laminae examined regardless of the incubation period.Downloads
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Published
2008-07-28
Issue
Section
Agronomy
DECLARAÇÃO DE ORIGINALIDADE E CESSÃO DE DIREITOS AUTORAIS
Declaro que o presente artigo é original, não tendo sido submetido à publicação em qualquer outro periódico nacional ou internacional, quer seja em parte ou em sua totalidade. Declaro, ainda, que uma vez publicado na revista Revista UNIMAR, editada pela Universidade Estadual de Maringá, o mesmo jamais será submetido por mim ou por qualquer um dos demais co-autores a qualquer outro periódico. Através deste instrumento, em meu nome e em nome dos demais co-autores, porventura existentes, cedo os direitos autorais do referido artigo à Universidade Estadual de Maringá e declaro estar ciente de que a não observância deste compromisso submeterá o infrator a sanções e penas previstas na Lei de Proteção de Direitos Autorias (Nº9609, de 19/02/98)