<b>Quantification and analysis of the viability of (oo)cysts of pathogenic protozoa in sewage sludge

Autores

  • Raphael Corrêa Medeiros Universidade Federal de Santa Maria
  • Luiz Antonio Daniel Universidade de São Paulo

DOI:

https://doi.org/10.4025/actascitechnol.v40i1.28709

Palavras-chave:

Cryptosporidium, Giardia, activated sludge, reuse.

Resumo

For the use of sewage sludge, it is extremely important to consider the microbiological aspect of this byproduct that poses direct and indirect risks to public health as for its inadequate handling and use. This study aimed to quantify Giardia spp. cysts and Cryptosporidium spp. oocysts and infer their viability in sludge samples from two sewage treatment plants. The method used consisted of three successive centrifugations followed by immunofluorescence assay and staining with propidium iodide. Samples were 100% (14/14) positive for cysts and 57.1% (8/14) for oocysts, with higher concentrations and mean viability above 75%. The change in the treatment of activated sludge, from extended aeration to conventional system, caused no difference in the concentration and viability of (oo)cysts.

 

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Biografia do Autor

Raphael Corrêa Medeiros, Universidade Federal de Santa Maria

Professor Adjunto do Departamento de Ciências Agronômicas e Ambientais, na área de Saneamento Ambiental, dentro do curso de Engenharia Ambiental e Sanitária

Luiz Antonio Daniel, Universidade de São Paulo

Professor Doutor da Escola de Engenharia de São Carlos, Departamento de Hidráulica e Saneamente, área de Saneamento.

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Publicado

2018-01-01

Como Citar

Medeiros, R. C., & Daniel, L. A. (2018). <b>Quantification and analysis of the viability of (oo)cysts of pathogenic protozoa in sewage sludge. Acta Scientiarum. Technology, 40(1), e28709. https://doi.org/10.4025/actascitechnol.v40i1.28709

Edição

Seção

Engenharia Civil

 

0.8
2019CiteScore
 
 
36th percentile
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0.8
2019CiteScore
 
 
36th percentile
Powered by  Scopus