Bioactive compounds in the leaves of Baccharis dracunculifolia: extraction process and characterization

Autores

  • Genice Iurckevicz Universidade Estadual do Centro Oeste https://orcid.org/0000-0002-5367-2113
  • Débora Dahmer Universidade Tecnológica Federal do Paraná
  • Maira Casagrande Universidade Estadual do Centro Oeste
  • Gabrielle Cristina Calegari Universidade Tecnológica Federal do Paraná
  • Tatiane Luiza Cadorin Oldoni Universidade Tecnológica Federal do Paraná
  • Robert Frans Huibert Dekker Universidade Tecnológica Federal do Paraná
  • Carlos Ricardo Maneck Malfatti Universidade Estadual do Centro Oeste
  • Mário Antônio Alves Cunha Universidade Tecnológica Federal do Paraná

DOI:

https://doi.org/10.4025/actascitechnol.v43i1.49826

Palavras-chave:

biological activity; experimental design; leaf extracts; natural products.

Resumo

Baccharis dracunculifolia has been used in popular medicine because of its bioactive properties, which include anti-inflammatory, antiulcerative and hepatoprotective potentials. A 2³ central composite rotational design was used to define the best conditions for extracting the bioactive compounds from the leaves of Baccharis dracunculifolia. The parameters studied included the solvent concentration, time and temperature of extraction and the influence of these on the total phenolics content and antioxidant activity. The best conditions were 95 % methanol (v/v), 20 min extraction time, and 70 °C temperature. Under the optimized conditions, high contents of total phenolic compounds (72.5 mg GAE g-1) and high DPPH (865.6 µmol TE g-1) and ABTS (331.11 μmol TE g-1) free radical scavenging capacity and a high ferric reducing antioxidant power (1558.00 μmol FeSO4.7H2O g-1), were obtained. The extract also showed promising anti-microbial activity, demonstrating fungistatic action against C. tropicalis and C. albicans (5 mg mL-1 and 7 mg mL-1, respectively) and bacteriostatic effect against Escherichia coli (60 e 70 mg mL-1), Salmonella enterica typhimurium (40; 60 and 70 mg mL-1) and Staphylococcus aureus (4 mg mL-1). HPLC-DAD analysis showed an extract rich in the phenolic compounds catechin (0.659 mg g-1), caffeic acid (1.135 mg g-1), p-coumaric acid (0.772 mg g-1), ferulic acid (6.24 mg g-1), pinocembrina (0.155 mg g-1) and rutin (0.037 mg g-1).

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Publicado

2021-02-26

Como Citar

Iurckevicz, G., Dahmer, D., Casagrande, M. ., Calegari, G. C. ., Oldoni, T. L. C. ., Dekker, R. F. H. ., Malfatti, C. R. M. ., & Cunha, M. A. A. . (2021). Bioactive compounds in the leaves of Baccharis dracunculifolia: extraction process and characterization. Acta Scientiarum. Technology, 43(1), e49826. https://doi.org/10.4025/actascitechnol.v43i1.49826

Edição

Seção

Biotecnologia

 

0.8
2019CiteScore
 
 
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0.8
2019CiteScore
 
 
36th percentile
Powered by  Scopus

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