Bioactive compounds from Syzygium malaccense leaves: optimization of the extraction process, biological and chemical characterization

Resumo

Syzygium malaccense belongs to the family Myrtaceae and it is popularly recognised as `jambo´ in Brazil. It is poorly studied regarding the bioactive compounds of fruits and leaves. This study aimed at optimizing the extraction and characterization of bioactives compounds from S. malaccense leaves. Extraction was optimized using a 23 full factorial design to evaluate the effect of ethanol concentration, time and temperature on polyphenols and antioxidants contents. Analyses of phenolic compounds content (Folin-Ciocalteau and HPLC-DAD), in vitro antioxidant capacities (DPPH, ABTS and FRAP), antimicrobial activity against bacteria and yeasts of ethanolic extract was carried out in optimal conditions of extraction. The optimized extraction condition was 45 min., 80°C and 40:60 (v v^-1) ethanol:water. The extract showed high total phenolic content (0.074 mg GAE kg^-1), DPPH (0.666 μmol TE kg^-1) and ABTS (0.853 μmol TE kg^-1) free radical scavenging ability and appreciable ferric reducing power (1.267 μmol Fe²⁺ kg^-1). HPLC methodology allowed the identification of four bioactive compounds: gallic acid (0.00036 mg kg^-1), catechin (0.00021 mg kg^-1), rutin (0.00027 mg kg^-1) and quercetin (0.00003 mg kg^-1). The extract showed inhibitory activity against S. aureus (1 µg L^-1); S. bongori (8 µg L^-1), C. albicans and C. tropicalis (2 µg L^-1). It also showed microbicidal potential against S. aureus; C. albicans and C. tropicalis.