<b>Quantification and analysis of the viability of (oo)cysts of pathogenic protozoa in sewage sludge

Raphael Corrêa Medeiros; Luiz Antonio Daniel

doi:10.4025/actascitechnol.v40i1.28709

<b>Quantification and analysis of the viability of (oo)cysts of pathogenic protozoa in sewage sludge

Authors

Raphael Corrêa Medeiros Universidade Federal de Santa Maria
Luiz Antonio Daniel Universidade de São Paulo

DOI:

https://doi.org/10.4025/actascitechnol.v40i1.28709

Keywords:

Cryptosporidium, Giardia, activated sludge, reuse.

Abstract

For the use of sewage sludge, it is extremely important to consider the microbiological aspect of this byproduct that poses direct and indirect risks to public health as for its inadequate handling and use. This study aimed to quantify Giardia spp. cysts and Cryptosporidium spp. oocysts and infer their viability in sludge samples from two sewage treatment plants. The method used consisted of three successive centrifugations followed by immunofluorescence assay and staining with propidium iodide. Samples were 100% (14/14) positive for cysts and 57.1% (8/14) for oocysts, with higher concentrations and mean viability above 75%. The change in the treatment of activated sludge, from extended aeration to conventional system, caused no difference in the concentration and viability of (oo)cysts.

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Author Biographies

Raphael Corrêa Medeiros, Universidade Federal de Santa Maria

Professor adjunto do Departamento de Ciências Agronômicas e Ambientais. Írea de Saneamento Ambiental

Luiz Antonio Daniel, Universidade de São Paulo

Professor Doutor do Departamento de Hidráulica e Saneamento. Escola de Engenharia de São Carlos.

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Published

2018-01-01

How to Cite

Medeiros, R. C., & Daniel, L. A. (2018). <b>Quantification and analysis of the viability of (oo)cysts of pathogenic protozoa in sewage sludge. Acta Scientiarum. Technology, 40(1), e28709. https://doi.org/10.4025/actascitechnol.v40i1.28709

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Issue

Vol. 40 (2018): Publicação Contínua

Section

Civil Engineering

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0.8

2019CiteScore

36th percentile