Effects of process conditions on exopolysaccharide produced by Mesorhizobium sp. in whey permeate
DOI:
https://doi.org/10.4025/actascitechnol.v48i1.74391Keywords:
Biopolymer; Diazotrophic bacteria; Experimental design.Abstract
Exopolysaccharides (EPS) produced by diazotrophic bacteria are promising biomolecules with commercial potential; however, effects of cultivation conditions on their yield have remained underexplored. This study aimed to maximize EPS production by Mesorhizobium sp. SEMIA 816 in whey permeate (WP) as the carbon source. A Plackett–Burman (PB) design was applied to assess the impact of 12 variables, namely K?HPO?, KH?PO?, MgSO?·7H?O, NaCl, yeast extract (YE), MnCl?·4H?O, CaCl?·2H?O, WP, pH, medium-to-reactor volume ratio (VM:VR), agitation and temperature, on EPS and biomass concentrations. EPS production ranged from 0 to 9.28 g L-1; the WP concentration exerted the most positive influence. Biomass production ranged from 0.6 to 7.15 g L-1; YE exerted the greatest effect, although it was negatively correlated with EPS production. Maximum EPS concentration (9.28 g L-1) was achieved after 96 h under the central point conditions of the experimental design, whereas the highest biomass concentration (7.15 g L-1) was reached after 72 h under a different set of conditions. Agitation and temperature influenced both responses negatively, a fact that highlighted the need to control them precisely. This study provides insights into the maximization of EPS production since it shows the potential of WP as an effective carbon source and identifies key factors affecting both EPS and biomass yields.
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