Biodegradation of a textile dye by Ganoderma lucidum: scale-up into packed-bed bioreactors

Juliane Andressa  Chicatto; Marcel Jefferson  Gonçalves; Thaynã Gonçalves  Timm; Cristiane Vieira  Helm; Deisi Altmajer-Vaz; Lorena Benathar Ballod  Tavares

doi:10.4025/actascitechnol.v48i1.74839

Authors

Juliane Andressa Chicatto Universidade Regional de Blumenau https://orcid.org/0000-0001-5811-0614
Marcel Jefferson Gonçalves Universidade Regional de Blumenau https://orcid.org/0000-0001-9511-9546
Thaynã Gonçalves Timm Universidade Regional de Blumenau https://orcid.org/0000-0001-5683-3536
Cristiane Vieira Helm Empresa Brasileira de Pesquisa Agropecuária https://orcid.org/0000-0002-7518-9069
Deisi Altmajer-Vaz University of Granada https://orcid.org/0000-0002-0866-9708
Lorena Benathar Ballod Tavares Universidade Regional de Blumenau https://orcid.org/0000-0001-7633-8196

DOI:

https://doi.org/10.4025/actascitechnol.v48i1.74839

Keywords:

Bactris gasipaes; discolorization; ganoderma lucidum; ligninolytic enzymes; peach palm waste; solid-state fermentation.

Abstract

This study presents the scale-up of the discoloration process of the dye Remazol Brilliant Blue R (RBBR) by the white-rot fungus Ganoderma lucidum. Experiments were initially conducted in a 2-L lab-scale bioreactor and subsequently scaled up to a 45-L packed-bed bioreactor operated in batch mode under nonsterile conditions. The dye solution (150 mg L^-1) was recirculated through the packed bed, which supported the growth of G. lucidum on an inert and biodegradable carrier — peach palm waste. Discoloration, enzyme activity, pH, and microbial community composition were analyzed. In the lab-scale system, discoloration reached 80%, with laccase activity measured at 847.2 IU mL^-1. Upon scale-up, discoloration reached 91%, with laccase identified as the main factor contributing to biodecoloration. The bacterial community was initially dominated by the phylum Proteobacteria (93.4%), followed by an increase in Acidobacteria, Actinobacteria, and Bacteroidetes. Among eukaryotes, Opisthokonta represented over 96% of the community. These results suggest that G. lucidum is a promising agent for the industrial-scale biodegradation of RBBR dye.

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