The Use of Supercritical CO? with Additives to Enhance Starch-Digesting Enzyme Activities in Bacillus subtilis
DOI:
https://doi.org/10.4025/actascitechnol.v48i1.74265Palavras-chave:
mutation breeding, dimethyl sulfoxide, hydrogen peroxide, spore.Resumo
Supercritical carbon dioxide (SC-CO2) has been proposed as a sterilization technique and utilized as a breeding method to enhance the activity of enzymes produced by microorganisms by treating their vegetative cells. This study explores whether this breeding method could be applicable to bacterial spores. Spores of Bacillus subtilis were treated with SC-CO2 in the presence of additives such as dimethyl sulfoxide (DMSO) or hydrogen peroxide (H2O2). Various concentrations of these additives were employed to screen for strains exhibiting increased starch-digesting enzyme activity. The resulting strains, D2-5 and H2-1, demonstrated improved starch-digesting enzyme activities after SC-CO2 treatment with 2% DMSO or 0.6% H2O2, respectively, which were 74.7% and 67.3% higher than the wild-type strain. Eight successive subcultures of these two strains indicated their hereditary stability. The findings demonstrated that SC-CO2, in combination with specific additives, could effectively increase the activity of enzymes produced by microorganisms when their spores were treated.
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