Selectivicty of Lecanicillium lecanii and Metarhizium anisopliae for Ceraeochrysa cincta first instar larvae (Neuroptera: Chrysopidae) in laboratory - DOI: 10.4025/actasciagron.v29i4.436

Abstract

This paper aimed to evaluate the selectivity of the fungi Lecanicillium lecanii and Metarhizium anisopliae for Ceraeochrysa cincta. Two bioassays were developed: 1^st) application of dry film proposed by the International Organization for Biological Control IOBC (modified) - they were applied in glass bases, forming dry film. 2^nd) immersion application. In the two methods, the following treatments were applied: 1- watery suspension of L. lecanii; 2- the same for M. anisopliae (both at 2.1x107 viable conidia mL-1); and 3- Tween 80^®0.05% watery solution. The larvae mortality, the average duration of the larvae period, the females fecundity and the eggs viability were evaluated. All the dead insects were submitted to asepsis to confirm the mortality caused by the fungi. In both bioassays, the mortality caused by the fungi was not verified. In the bioassay dry film, the fungi total effect on the predator was classified as not very harmful according to the categories proposed by the IOBC method. L. lecanii and M. anisopliae did not affect the viability of C. cincta eggs, when applied by immersion. In the established concentration for the accomplishment of the bioassays, entomopatogenic fungi L. lecanii and M. anisopliae were selective for first instar larvae of C. cincta