Transformação genética de citrumelo Swingle (Citrus paradisi Macf. × Poncirus trifoliata L. Raf.) com o gene marcador fosfomanose isomerase (pmi) - DOI: 10.4025/actasciagron.v27i3.1473

José Geraldo Zaparoli Vieira; Ricardo Tadeu de Faria; Luiz Gonzaga Esteves Vieira; Hugo Bruno Correa Molinari

doi:10.4025/actasciagron.v27i3.1473

Genetic transformation of Swingle citrumelo (Citrus paradisi Macf. x Poncirus trifoliata L. Raf.) with the marker gene phosphomannose isomerase (pmi) - DOI: 10.4025/actasciagron.v27i3.1473

José Geraldo Zaparoli Vieira UEL
Ricardo Tadeu de Faria UEL
Luiz Gonzaga Esteves Vieira IAPAR
Hugo Bruno Correa Molinari IAPAR

DOI: https://doi.org/10.4025/actasciagron.v27i3.1473

Abstract

A transformation protocol using mannose as selective agent was evaluated for genetic transformation of the rootstock Swingle citrumelo. Two independent experiments were carried out to define the smallest mannose concentration capable to inhibit the multiplication of adventitious buds. Based on these results, 5.0 mM of mannose was chosen for Swingle citrumelo genetic transformation. An 11% transformation efficiency was obtained using the Agrobacterium tumefaciens binary strain EHA105. The results described in this study showed that, despite the relatively low transformation frequency, the gene pmi is a good alternative to the most commonly used selection scheme for citrus transformation, based on gene nptII for kanamycin resistance.

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Author Biography

Ricardo Tadeu de Faria, UEL

Possui graduação em Agronomia pela Faculdade de Agronomia e Zootecnia Manoel Carlos Gonçalves (1987) , mestrado em Genética e Biologia Molecular pela Universidade Estadual de Campinas (1992) , doutorado em Genética e Biologia Molecular pela Universidade Estadual de Campinas (1998) e pos-doutorado pela Universidade de La Laguna (2003) . Atualmente é Associado A da Universidade Estadual de Londrina, Revisor de periódico da Acta Scientiarum (UEM), Revisor de periódico da Semina Ciência Agrária, Revisor de periódico da Revista Brasileira de Horticultura Ornamental, Revisor de periódico da Horticultura Brasileira, Revisor de periódico da Magistra, Revisor de periódico da Magistra, Revisor de periódico da Crop Breeding and Applied Biotechnology e Revisor de periódico da Ciência e Agrotecnologia. Tem experiência na área de Agronomia , com ênfase em Floricultura, Parques e Jardins Currículo Lattes

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Published

2008-03-27

How to Cite

Vieira, J. G. Z., Faria, R. T. de, Vieira, L. G. E., & Molinari, H. B. C. (2008). Genetic transformation of Swingle citrumelo (Citrus paradisi Macf. x Poncirus trifoliata L. Raf.) with the marker gene phosphomannose isomerase (pmi) - DOI: 10.4025/actasciagron.v27i3.1473. Acta Scientiarum. Agronomy, 27(3), 541-547. https://doi.org/10.4025/actasciagron.v27i3.1473

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Issue

Vol 27 No 3 (2005)

Section

Genetics and Plant Breeding

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