Atividade celulolítica in vitro de Cylindrocladium clavatum e o efeito de filtrados de cultivo do fitopatógeno sobre plântulas de eucalipto - DOI: 10.4025/actasciagron.v25i1.2589

Kátia Regina Freitas Schwan-Estrada; José Renato Stangarlin; Ana Cristina Grade Fiori; Sérgio Florentino Pascholati

doi:10.4025/actasciagron.v25i1.2589

The in vitro production of Cellulolytic activiy and the effect of Cylindrocladium clavatum in Eucalyptus urophylla seedlings - DOI: 10.4025/actasciagron.v25i1.2589

Kátia Regina Freitas Schwan-Estrada Universidade Estadual de Maringá, Centro de Ciências Agrárias, Agronomia
José Renato Stangarlin UNIOESTE
Ana Cristina Grade Fiori UEM
Sérgio Florentino Pascholati USP

DOI: https://doi.org/10.4025/actasciagron.v25i1.2589

Abstract

Under nursery conditions, the diseases which mostly affect the Eucalyptus seedlings are damping-off and cutting rot for rooting, caused by Cylindrocladium clavatum. The mechanism of attack of this pathogen is not completely understood, but it is known that phytotoxins and exoenzymes, produced by phytopathogenic fungi, are important in host penetration and colonization. Thus, the objectives of this investigation were to demonstrate the in vitro production of cellulolytic enzymes and toxic metabolites of C. clavatum in E. urophylla seedlings. Results showed that cellulase produced by fungus is a multienzymatic complex consisting of two endo and three exo ß-1,4 glucanases and one ß-glucosidase, and that C. clavatum can produce such in vitro enzymes when cultivated in different substrates. The presence of toxic metabolites, related to disease symptoms appearance in E. urophylla seedlings was also observed.

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Author Biography

Kátia Regina Freitas Schwan-Estrada, Universidade Estadual de Maringá, Centro de Ciências Agrárias, Agronomia

possui graduação em Agronomia pela Universidade Federal do Espírito Santo (1979), mestrado em Microbiologia Agrícola pela Universidade Federal de Viçosa (1984) e doutorado em Fitopatologia pela Escola Superior de Agricultura Luiz de Queiroz (1996). Atualmente é professor associado da Universidade Estadual de Maringá; orientador de mestrado e doutorado; Assessor adhoc Acta Scientiarum (UEM), Ciência e Agrotecnologia , Fitopatologia Brasileira e Summa Phytopathologica. Tem experiência na área de Agronomia, com ênfase em Fisiologia do Parasitismo, atuando principalmente nos seguintes temas: controle alternativo, planta medicinal, fungos e indução de resistência. http://lattes.cnpq.br/7333463527916515

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Published

2008-04-23

How to Cite

Schwan-Estrada, K. R. F., Stangarlin, J. R., Fiori, A. C. G., & Pascholati, S. F. (2008). The in vitro production of Cellulolytic activiy and the effect of Cylindrocladium clavatum in Eucalyptus urophylla seedlings - DOI: 10.4025/actasciagron.v25i1.2589. Acta Scientiarum. Agronomy, 25(1), 155-160. https://doi.org/10.4025/actasciagron.v25i1.2589

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Issue

Vol 25 No 1 (2003)

Section

Agronomy

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