<b>Quantitative analysis of rumen microbial populations by qPCR in heifers fed on <i>Leucaena leucocephala</i> in the Colombian Tropical Dry Forest

Erika Angarita; Isabel Molina; Gonzalo Villegas; Olga Mayorga; Julián Chará; Rolando Barahona Rosales

doi:10.4025/actascianimsci.v37i2.24836

Erika Angarita Colombian Corporation of Agricultural Research Corpoica / Center for Research on Sustainable Farming Systems
Isabel Molina National University of Colombia / Center for Research on Sustainable Farming Systems
Gonzalo Villegas Center for Research on Sustainable Farming Systems
Olga Mayorga Colombian Corporation of Agricultural Research Corpoica
Julián Chará Center for Research on Sustainable Farming Systems
Rolando Barahona Rosales National University of Colombia

DOI: https://doi.org/10.4025/actascianimsci.v37i2.24836

Resumo

Rumen fermentation and methanogenesis are vital metabolic processes in cattle and are carried out by microbial populations that are affected by dietary factors such as secondary metabolites, nutritional composition and degradability. The aim of this study was to monitor populations of total bacteria, total methanogens and Butirivibrio fibrisolvens in the rumen of Lucerne heifers fed on diets typical of intensive silvopastoral systems (ISS) or of a traditional (control) system. Rumen contents (RC) were collected orally from eight heifers consuming 100% Cynodon plectostachyus (control) and 76% C. plectostachyus + 24% Leucaena leucocephala (ISS) following a crossover design and DNA was extracted and quantified by quantitative PCR. Populations [Log10 (ng g^-1 RC)] were 5.6 and 5.8 for total bacteria (p = 0.5343), 3.6 and 3.5 for B. fibrisolvens (p = 0.4742) and 5.0 and 5.3 for total methanogens (p = 0.2661) respectively in control and ISS diets. However, when measured in a separate parallel study, enteric methane emissions (g kg^-1 of fermented dry matter) were significantly reduced with the inclusion of L. leucocephala. This fact indicated the importance of investigating the structure, function and interactions of populations beyond quantitative analysis to determine how diet affects rumen microbial populations and their function.

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Biografia do Autor

Erika Angarita, Colombian Corporation of Agricultural Research Corpoica / Center for Research on Sustainable Farming Systems

Zootecnista, investigadora y asesora.

Isabel Molina, National University of Colombia / Center for Research on Sustainable Farming Systems

Zootecnista.

Gonzalo Villegas, Center for Research on Sustainable Farming Systems

Zootecnista.

Olga Mayorga, Colombian Corporation of Agricultural Research Corpoica

Investigador Ph.D.

Julián Chará, Center for Research on Sustainable Farming Systems

Investigador Ph.D.

Rolando Barahona Rosales, National University of Colombia

Investigador Ph.D. y profesor

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