<b>Study of the genetic polymorphism of the α_S1-casein in goats of Pernambuco State, Brazil</b> - DOI: 10.4025/actascianimsci.v29i3.551

Abstract

The Pernambuco State, has been a livestock area, mainly for the caprine exploration. Among the proteins, called caseins, the α_S1-casein was the first proved protein with base in the genetic polymorphism. To genotype goats of the “sertão”, “agreste” and “zona da mata” regions of the Brazilian State of Pernambuco, through the PCR-RFLP technique, we studied the polymorphism of the α_S1-casein gene. Sixty animals were used, divided in three groups of twenty animals of the races Moxotó, American Alpine and UB (Undefined Breed). The DNA extraction was done by the phenol-chloroform protocol and the α_S1-casein gene was amplified through the PCR (Polymerase Chain Reaction). Then, the restriction enzyme XmnI was used to obtain the allele frequency of the studied races. In the goats, we found the α_S1-casein alleles B and D that predominantly were in the native race Moxotó and Ub animals (100%), the alleles, C and D for the American Alpine race (100%), concluding that there are genetic variations for the α_S1-casein in the milk of the goat races studied in the region, although it was observed a genetic proximity between Moxotó and UB.