Effect of intraspecies interactions on Pseudomonas aeruginosa biofilm formation and gene expression

  • Mariana Alcântara Calomino Universidade Federal Fluminense
  • Michelle Pereira Silva Universidade Federal Fluminense
  • Heidi Pauer The University of Kansas
  • Beatriz Guitton Renaud Baptista de Oliveira Universidade Federal Fluminense
  • Francislene Juliana Martins Universidade Federal Fluminense
  • Gabriel Luis Cavalcanti Valente Universidade Federal Fluminense
  • Geraldo Renato de Paula Universidade Federal Fluminense
  • Felipe Lopes Teixeira Universidade Federal Fluminense / The University of Kansas
Palavras-chave: Iron uptake modulation; production of biofilms; quorum sensing; pyoverdine; secondary metabolites.

Resumo

Pseudomonas aeruginosa is a common pathogen of acute and chronic infections that can increase risk to burned patients and those with chronic wounds, especially when forming biofilms. These complex ecosystems are formed by several bacteria that communicate, cooperate, and compete with each other.  To enhance its survival capacity in such a competitive environment, P. aeruginosa produces secondary metabolites with a range of biological activities, among other factors. Here, we evaluated how such bioactive molecules mediate intraspecies interactions to modulate P. aeruginosa biofilm formation. An overall decrease in biofilm formation on the surface of stainless steel coupons was observed for both dual-strain and single-strain biofilms formed in the presence of secondary metabolites extracted from other P. aeruginosa strains. Modulation of biofilm formation by these metabolites during P. aeruginosa intraspecies interactions suggests a role for these molecules in strain competition. The most likely mechanism by which it occurs might be modulation of iron uptake. Among the genes analyzed, those related to import (tonB1) and biosynthesis (pvdS) of the siderophore pyoverdine were the most downregulated during exposure to the metabolite extracts. Exploring P. aeruginosa intraspecies interactions during biofilm formation might be an interesting approach to understand its lifestyle better and, thus, the mechanisms involved in the species’ biofilm formation.

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Publicado
2024-08-27
Como Citar
Calomino, M. A., Silva, M. P., Pauer, H., Oliveira, B. G. R. B. de, Martins, F. J., Valente, G. L. C., Paula, G. R. de, & Teixeira, F. L. (2024). Effect of intraspecies interactions on Pseudomonas aeruginosa biofilm formation and gene expression . Acta Scientiarum. Biological Sciences, 46(1), e70172. https://doi.org/10.4025/actascibiolsci.v46i1.70172
Seção
Microbiologia

 

0.6
2019CiteScore
 
 
31st percentile
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0.6
2019CiteScore
 
 
31st percentile
Powered by  Scopus