Genetic variability in Oligosarcus paranensis (Teleostei: Characiformes) from the São Francisco river, Ivaí river basin – Paraná State, Brazil - doi: 10.4025/actascibiolsci.v35i3.14179

Michele Rocha dos Santos; Erasmo Renesto

doi:10.4025/actascibiolsci.v35i3.14179

Genetic variability in Oligosarcus paranensis (Teleostei: Characiformes) from the São Francisco river, Ivaí river basin – Paraná State, Brazil - doi: 10.4025/actascibiolsci.v35i3.14179

Michele Rocha dos Santos Universidade Estadual de Maringá
Erasmo Renesto Universidade Estadual de Maringá

DOI: https://doi.org/10.4025/actascibiolsci.v35i3.14179

Abstract

The genetic variability of Oligosarcus paranensis was estimated from a population collected in São Francisco river, Prudentópolis county in Paraná State (Brazil) using the electrophoresis in starch gel technique. Eleven enzymatic systems were analyzed: Aspartate aminotransaminase (AAT; E. C. 2.6.1), Alcohol dehydrogenase (ADH; E. C. 1.1.1.1), Esterase (EST; E. C. 3.1.1.1), Glucose-6-phosphate isomerase (GPI; E. C. 5.3.1.9), Glycerol-3-Phosphate dehydrogenase (G3PDH; E. C. 1.1.1), Isocitrate dehydrogenase (IDH; E. C. 1.1.1.42), L-lactate dehydrogenase (LDH; E. C. 1.1.1.27), Malate dehydrogenase (MDH; E. C. 1.1.1.37 ), Malate dehydrogenase NADP (ME; E. C. 1.1.1.40), Phosphoglucomutase (PGM; E. C. 5.4.2.2) and Sorbitol dehydrogenase (SORB; E.C. 1.1.1.14). Twenty loci were identified through 15% corn starch gel electrophoresis of which nine (45%) were polymorphic. The average expected heterozygosity was estimated as 0.1229 ± 0.1728, and the observed was 0.0586 ± 0.1069, indicating high genetic variability. The average value of F_IS = 0.5145 indicates homozygote excess.

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Author Biography

Michele Rocha dos Santos, Universidade Estadual de Maringá

Graduada em Ciências Biológicas, Mestre em Genética e Melhoramento pela Universidade Estadual de Maringá - UEM e professora do Ensino Médio pelo Estado do Paraná.

Published

2013-02-28

How to Cite

Santos, M. R. dos, & Renesto, E. (2013). Genetic variability in Oligosarcus paranensis (Teleostei: Characiformes) from the São Francisco river, Ivaí river basin – Paraná State, Brazil - doi: 10.4025/actascibiolsci.v35i3.14179. Acta Scientiarum. Biological Sciences, 35(3), 389-394. https://doi.org/10.4025/actascibiolsci.v35i3.14179

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Issue

Vol 35 No 3 (2013)

Section

Genetics

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