Efeito de combinações diferentes de reguladores de crescimento a partir da indução de botões de plântulas de Cattleya walkeriana Gardner (Orchidaceae) - DOI: 10.4025/actascibiolsci.v25i1.2106

Priscila Vanessa Krapiec; Maria Auxiliadora Milaneze; Maria de Fátima Pires da Silva Machado

doi:10.4025/actascibiolsci.v25i1.2106

Effects of different combinations of growth regulators for bud induction from seedlings of Cattleya walkeriana Gardner (Orchidaceae) - DOI: 10.4025/actascibiolsci.v25i1.2106

Priscila Vanessa Krapiec UEM
Maria Auxiliadora Milaneze UEM
Maria de Fátima Pires da Silva Machado Biologia - UEM

DOI: https://doi.org/10.4025/actascibiolsci.v25i1.2106

Abstract

The present study reports a research with different concentrations and combinations of auxins and cytokinins to activate bud development and to obtain multiplication of Cattleya walkeriana (Orchidaceae) without an intermediate callus stage. Seedlings of C. walkeriana were cultured on B5 medium containing 2% sucrose and solidified with 0.65% agar, with factorial combinations of the auxins 1-naphthaleneacetic acid (NAA), 3-indolbutyric acid (IBA), and cytokinins 6-benzylaminopurine (BA) and N-(2-furanyl-methyl)-1-purine-6 amine (Kinetin) at concentrations of 0.0, 0.5, 1.0, and 1.5 mg/L. The highest number of buds with elongated leaves occurred by cultivating seedlings on medium containing different concentrations of IBA and BA, while the lowest number was observed in NAA and KIN combination. IBA alone yielded better results at the three tested concentrations, when compared to the total absence of growth regulators. The higher frequency of bud induction using BA and IBA combination indicated that this combinations were more effective for C. walkeriana in vitro multiplication using seedlings as explants

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Author Biography

Maria de Fátima Pires da Silva Machado, Biologia - UEM

Possui graduação em Licenciatura Em Ciências Biológicas pela Universidade de São Paulo (1978) , mestrado em Ciências Biológicas (Biologia Genética) pela Universidade de São Paulo (1982) , doutorado em Ciências Biológicas (Biologia Genética) pela Universidade de São Paulo (1986) e pos-doutorado pelo Instituto de Biologia Celular de Madrid C S I C (1989) . Atualmente é Professor Associado da Universidade Estadual de Maringá, Revisor de periódico da Ciência Rural e Revisor de periódico da Acta Scientiarum (UEM). Tem experiência na área de Genética , com ênfase em Genética Animal. Atuando principalmente nos seguintes temas: G-3-PDH (EC 1.1.1.8), ISOENZIMAS, HIMENOPTEROS. Currículo Lattes

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Published

2008-04-18

How to Cite

Krapiec, P. V., Milaneze, M. A., & Machado, M. de F. P. da S. (2008). Effects of different combinations of growth regulators for bud induction from seedlings of Cattleya walkeriana Gardner (Orchidaceae) - DOI: 10.4025/actascibiolsci.v25i1.2106. Acta Scientiarum. Biological Sciences, 25(1), 179-182. https://doi.org/10.4025/actascibiolsci.v25i1.2106

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Issue

Vol 25 No 1 (2003)

Section

Biology Sciences

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