Extraction, physical-chemical characterization and in vitro inhibitory potential of thrombin generation of crude sulfated polysaccharides from Brazilian tropical seaweeds

José Ariévilo Gurgel Rodrigues; Priscila Gomes Barcellos; Bianca Fernandes Glauser; Ana Luíza Gomes Quinderé; Norma Maria Barros Benevides; Ana Maria Freire Tovar; Paulo Antônio de Souza Mourão

doi:10.4025/actascibiolsci.v40i1.34463

Extraction, physical-chemical characterization and in vitro inhibitory potential of thrombin generation of crude sulfated polysaccharides from Brazilian tropical seaweeds

José Ariévilo Gurgel Rodrigues Universidade Federal do Rio de Janeiro
Priscila Gomes Barcellos Universidade Federal do Rio de Janeiro
Bianca Fernandes Glauser Universidade Federal do Rio de Janeiro
Ana Luíza Gomes Quinderé Universidade Federal do Ceará
Norma Maria Barros Benevides Universidade Federal do Ceará
Ana Maria Freire Tovar Universidade Federal do Rio de Janeiro
Paulo Antônio de Souza Mourão Universidade Federal do Rio de Janeiro

DOI: https://doi.org/10.4025/actascibiolsci.v40i1.34463

Keywords: marine algae, polyglycans, coagulation tests, thrombin.

Abstract

The biotechnological value of macroalgae for screening assays of thrombin generation-TG using sulfated polysaccharides-SPs as substitutes to heparin has been poorly explored. Five Brazilian species of macroalgae (Gracilaria birdiae, Acanthophora muscoides, Halymenia sp., Caulerpa cupressoides and C. racemosa) were analyzed and compared for their abundance, physical-chemical characteristics and in vitro anticoagulant assays of activated partial thromboplastin time-APTT, prothrombin time-PT and TG. Papain extraction yielded (p < 0.001) from 0.66±0.03% (C. racemosa) to 41.60±1.10% (Halymenia sp.) of crude SPs varying sulfate (8.41-42.60%) and total sugars (47.80-70.53%). Crude SPs showed difference in mobility and resolution pattern by agarose electrophoresis, while polyacrylamide analysis revealed SPs of > 100 kDa. These procedures, combined with the use of Stains-All, also indicated nonSPs. APTTs ranged from 2.81 (A. muscoides) to 21.30 IU (Halymenia sp.) vs. heparin (193 IU), and were dependent on sulfation of the crude SPs. PT was not altered. With respect to TG assay, crude SPs modified concentration-dependent and independently from molecular mass TG by both intrinsic/extrinsic pathways in 60-fold diluted human plasma, with total intrinsic inactivation using crude SPs from A. muscoides in parallel to heparin (p < 0.05). Thrombosis in vitro is differentially modulated by distinct crude SPs from Brazilian seaweeds.

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Author Biography

José Ariévilo Gurgel Rodrigues, Universidade Federal do Rio de Janeiro

Graduado em Engenharia de Pesca pela Universidade Federal do Ceará (2004), mestrado em Recursos Pesqueiros e Engenharia de Pesca pela Universidade Federal do Ceará (2006) e doutorado em Biotecnologia pela Rede Nordeste de Biotecnologia - RENORBIO/UFC (2011). Tem experiência na área de Recursos Pesqueiros e Engenharia de Pesca e de Biotecnologia em Recursos Naturais, com ênfase em Taxonomia de Algas Marinhas, Fisiologia Comparada (taxonomia de grupos, filogenia e biogeografia) e Bioprospecçâo de Produtos Naturais Bioativos de Organismos Aquáticos. Atua nas seguintes áreas: Ficologia, Química de macromoléculas (polissacarídeos sulfatados) e suas propriedades químicas e implicações terapêuticas sobre coagulação, trombose, dor, inflamação e de avaliação toxicológica (aguda, subcrônica e crônica). Foi professor horista (prestador de serviços) do Instituto de Desenvolvimento, Educação e Cultura do Ceará (IDECC-UVA) e do Instituto Dom José de Educação e Cultura (IDJ-UVA). Atualmente, realiza Pós-Doutorado na Universidade Federal do Rio de Janeiro (CAPES-PNPD).

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Published

2018-03-28

How to Cite

Rodrigues, J. A. G., Barcellos, P. G., Glauser, B. F., Quinderé, A. L. G., Benevides, N. M. B., Tovar, A. M. F., & Mourão, P. A. de S. (2018). Extraction, physical-chemical characterization and in vitro inhibitory potential of thrombin generation of crude sulfated polysaccharides from Brazilian tropical seaweeds. Acta Scientiarum. Biological Sciences, 40(1), e34463. https://doi.org/10.4025/actascibiolsci.v40i1.34463

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Issue

Vol 40 (2018): Publicação Contínua

Section

Biotechnology

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