<b>Behavior of α-tomatine and tomatidine against several genera of trypanosomatids from insects and plants and <i>Trypanosoma cruzi

  • Adriane Feijó Evangelista Hospital de Câncer de Barretos
  • Erica Akemi Kavati Instituto Butantan
  • José Vitor Jankevicius Universidade Estadual de Londrina
  • Rafael Andrade Menolli Universidade Estadual do Oeste do Paraná

Resumo

 

Glycoalkaloids are important secondary metabolites accumulated by plants as protection against pathogens. One of them, α-tomatine, is found in high concentrations in green tomato fruits, while in the ripe fruits, its aglycone form, tomatidine, does not present a protective effect, and it is usual to find parasites of tomatoes like Phytomonas serpens in these ripe fruits. To investigate the sensitivity of trypanosomatids to the action of α-tomatine, we used logarithmic growth phase culture of 20 trypanosomatids from insects and plants and Trypanosoma cruzi. The lethal dose 50% (LD50) was determined by mixing 107 cells of the different isolates with α-tomatine at concentrations ranging from 10-3 to 10-8 M for 30 min at room temperature. The same tests performed with the tomatidine as a control showed no detectable toxicity against the same trypanosomatid cultures. The tests involved determination of the percentage (%) survival of the protozoan cultures in a Neubauer chamber using optical microscopy. The LD50 values varied from 10-4 to 10-6 M α-tomatine. Slight differences were detected among the LD50 values of the analyzed samples, and none of them showed evidence of resistance to the action of tomatinase, as shown by some pathogenic fungi.

 

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Biografia do Autor

Adriane Feijó Evangelista, Hospital de Câncer de Barretos

Centro de Pesquisa em Oncologia Molecular

Rua Antenor Duarte Vilela, 1331, CEP: 14784-400, Barretos, SP

Erica Akemi Kavati, Instituto Butantan

Laboratório de Genética

Avenida Vital Brazil, 1500, CEP: 05503-900, São Paulo, SP

José Vitor Jankevicius, Universidade Estadual de Londrina

Departamento de Microbiologia

Rodovia Celso Garcia Cid, Km 380, CEP: 86057-970, Londrina, PR

Rafael Andrade Menolli, Universidade Estadual do Oeste do Paraná
Laboratório de Imunologia Aplicada
Publicado
2018-11-30
Como Citar
Evangelista, A. F., Kavati, E. A., Jankevicius, J. V., & Menolli, R. A. (2018). <b>Behavior of α-tomatine and tomatidine against several genera of trypanosomatids from insects and plants and <i&gt;Trypanosoma cruzi. Acta Scientiarum. Biological Sciences, 40(1), e41853. https://doi.org/10.4025/actascibiolsci.v40i1.41853
Seção
Biotecnologia

 

0.6
2019CiteScore
 
 
31st percentile
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0.6
2019CiteScore
 
 
31st percentile
Powered by  Scopus