Utilização de linhagens diplóides uvsH//uvsH de Aspergillus nidulans (Ascomycetes) para a avaliação do potencial recombinagênico de agentes químicos e físicos

Cleverson Busso; Simone Jurema Ruggeri Chiuchetta; Francielle Baptista; Marialba Avezum Alves Castro Prado

doi:10.4025/actascibiolsci.v23i0.2718

uvsH//uvsH diploid strain favors an efficient method to evaluate the recombinagenic effect of chemical and physical agents in Aspergillus nidulans (Ascomycetes)

Authors

Cleverson Busso UEM Author
Simone Jurema Ruggeri Chiuchetta UEM Author
Francielle Baptista UEM Author
Marialba Avezum Alves Castro Prado UEM Author

DOI:

https://doi.org/10.4025/actascibiolsci.v23i0.2718

Keywords:

crossing-over mitótico, reparo do DNA, índice de homozigotização, genotoxicidade de substâncias químicas

Abstract

Ascomycete Aspergillus nidulans is an excellent system for mitotic crossing-over studies. This is due to the fact that much of its cell cycle is passed in G2 and presents uvs mutations that increase frequencies of normal mitotic recombinations (uvsF and uvsH). The aim of this research was to obtain a new diploid strain of A. nidulans with proper characteristics for recombinagenesis investigations, or rather, heterozygous for nutritional markers and conidia coloration and homozygous for uvsH mutation. Higher sensitivity of diploid uvsH//uvsH in the monitoring of mitotic recombination events was shown by higher indexes of the diploid’s spontaneous mitotic recombination when compared with diploid uvsH⁺//uvsH⁺. New strain is a versatile tool that may be used in different studies on mitotic recombination in A. nidulans

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Author Biography

Marialba Avezum Alves Castro Prado, UEM

possui graduação em Bacharelado Em Ciências Biológicas pela Organização Educacional Barão de Mauá (1980), mestrado em Ciências Biológicas (Genética) pela Universidade de São Paulo (1985) e doutorado em Ciências Biológicas (Genética) pela Universidade de São Paulo (1992). Atualmente é professor associado - nível c da Universidade Estadual de Maringá. Tem experiência na área de Genética, com ênfase em Controle da Expressão Gênica, atuando principalmente nos seguintes temas: Parassexualidade em fungos filamentosos, Genética do desenvolvimento, Controle do crossing-over e Genotoxicidade (recombinação mitótica) de substâncias químicas Currículo Lattes

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Published

2008-05-09

Issue

Vol. 23 (2001)

Section

Biology Sciences

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How to Cite

uvsH//uvsH diploid strain favors an efficient method to evaluate the recombinagenic effect of chemical and physical agents in Aspergillus nidulans (Ascomycetes). (2008). Acta Scientiarum. Biological Sciences, 23, 603-607. https://doi.org/10.4025/actascibiolsci.v23i0.2718