POTENTIAL ANTIDIABETIC OF TAMARIND WASTE: EFFECTS ON THE PANCREATIC -AMYLASE

Abstract

Diabetes is a complex metabolic disease characterized by plasma hyperglycemia. One of the
ways of treatment of diabetes is the inhibition of pancreatic α-amylase. However, drugs
available in the market carry out a range of side effects. Therefore, the present work aimed to
evaluate the effect of tamarind waste (skin and seed) rejected by the food industry on the
activity of pancreatic α-amylase. Hydroethanolic extract (70%) of tamarind skin and seed
were prepared, lyophilized, and used in a concentration curve on the α-amylase activity by 3,5
dinitro-salicylate assay. Kinetic analysis was performed simultaneously varying both substrate
(starch) and extracts. Statistical analyses were carried out by Scientist Program. Both extracts
(skin and seed) reduced the enzymatic activity in a concentration dependence way. However,
the seed extract reported a bigger strength of inhibition, as revealed by IC 50 of 13,26 µg/mL,
compared to IC 50 of 470,16 µg/mL for skin extract. Kinect studies showed that the seed
extract presented an enzymatic inhibition of parabolic and non-competitive type (KI 1 10,07
µg/mL e KI 2 10,37 µg/mL). Conversely, skin extract performed a mixed linear inhibition (KI 1
615,78 µg/mL e KI 2 411,01 µg/mL). We concluded that tamarind waste, mainly seed extract,
can be considered preparation with therapeutic potential for the management and treatment of
diabetes.