TENDÊNCIAS DE OTIMIZAÇÃO NA PRODUÇÃO DE LIPOPEPÍDEOS TOTAIS POR Bacillus velezensis 0G REVELARAM UM MÉTODO DE FERMENTAÇÃO SUBMERSO SUSTENTÁVEL USANDO CASCAS DE BATATA DOCE
Palavras-chave:
surfactina, otimização multivariada, superfície de resposta, Bacillus velezensis 0G
Resumo
Bacillus velezensis são microrganismos que promovem o crescimento das plantas. Eles produzem uma classe interessante de peptídeos cíclicos; surfactina, que possui propriedades antifúngicas e surfactantes. Os lipopeptídeos da família surfactina são biossurfactantes muito potentes, com importantes aplicações para remediação ambiental e indústrias químicas. No presente trabalho, a produção complexa de lipopeptídeo (principalmente surfactinas) por B. velezensis 0G foi avaliada sob diferentes condições de crescimento, utilizando-se o planejamento fatorial 23 com pontos centrais e axiais. Todas as experiências foram realizadas com base em fermentação submersa sustentável contendo cascas de batata-doce. As tendências da superfície de resposta demonstram uma produção máxima de biossurfactante em volumes de inóculo de 32 mL; volume combinado de caldo de 46mL e tempo de incubação de 24h. Estas observações foram baseadas na análise do sobrenadante por HPLC.
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Referências
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SYED, Shameer; CHINTHALA, Paramageetham. Heavy Metal Detoxification by Different Bacillus Species Isolated from Solar Salterns. Scientifica, v. 2015, p. 1–8, 2015. Disponível em:
TEÓFILO, Reinaldo F.; FERREIRA, Márcia M. C. Quimiometria II: planilhas eletrônicas para cálculos de planejamentos experimentais, um tutorial. Química Nova, v. 29, n. 2, p. 338–350, abr. 2006. Disponível em:. Acesso em: 18 dez. 2018.
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VECINO, Xanel et al. Sewage Sludge Polycyclic Aromatic Hydrocarbon (PAH) Decontamination Technique Based on the Utilization of a Lipopeptide Biosurfactant Extracted from Corn Steep Liquor. Journal of Agricultural and Food Chemistry, v. 63, n. 32, p. 7143–7150, 2015.
BAKRI, Yasser; MEKAEEL, Alaa; KOREIH, Amal. Influence of agitation speeds and aeration rates on the Xylanase activity of Aspergillus niger SS7. Brazilian Archives of Biology and Technology, v. 54, n. 4, p. 659–664, ago. 2011. Disponível em:
BEEBE, Kenneth R.; KOWALSKI, Bruce R. An Introduction to Multivariate Calibration and Analysis. Analytical Chemistry, v. 59, n. 17, 1987.
BEZERRA, Marcos Almeida et al. Response surface methodology (RSM) as a tool for optimization in analytical chemistry. Talanta, v. 76, n. 5, p. 965–977, 15 set. 2008. Disponível em:
BINIARZ, Piotr; ŁUKASZEWICZ, Marcin. Direct quantification of lipopeptide biosurfactants in biological samples via HPLC and UPLC-MS requires sample modification with an organic solvent. Applied Microbiology and Biotechnology, v. 101, n. 11, p. 4747–4759, 22 jun. 2017. Disponível em:
COOPER, D. G.; GOLDENBERG, B. G. Surface-active agents from two bacillus species. Applied and environmental microbiology, v. 53, n. 2, p. 224–9, fev. 1987. Disponível em:
DE AMORIM, Wellyngton Silva et al. The nexus between water, energy, and food in the context of the global risks: An analysis of the interactions between food, water, and energy security. Environmental Impact Assessment Review, v. 72, n. June 2017, p. 1–11, 2018. Disponível em:
DE, Sourav et al. A review on natural surfactants. RSC Advances, v. 5, n. 81, p. 65757–65767, 2015. Disponível em:
ETCHEGARAY, Augusto et al. Production of Bacillus amyloliquefaciens OG and its metabolites in renewable media: valorisation for biodiesel production and p -xylene decontamination. Canadian Journal of Microbiology, v. 63, n. 1, p. 46–60, jan. 2017. Disponível em:
GARG, Mayank; PRIYANKA; CHATTERJEE, Mary. Isolation, characterization and antibacterial effect of biosurfactant from Candida parapsilosis. Biotechnology Reports, v. 18, p. e00251, 1 jun. 2018. Disponível em:
HEUSON, Egon et al. Screening of Lipopeptide‐Producing Strains of Bacillus sp. Using a New Automated and Sensitive Fluorescence Detection Method. Biotechnology Journal, v. 14, n. 4, p. 1800314, 26 abr. 2019. Disponível em:
HMIDET, Noomen et al. Enhancement of Surfactin and Fengycin Production by Bacillus mojavensis A21: Application for Diesel Biodegradation. BioMed Research International, v. 2017, 2017.
JEMIL, Nawel et al. Structural characterization and identification of cyclic lipopeptides produced by Bacillus methylotrophicus DCS1 strain. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, v. 1060, n. June, p. 374–386, 2017. Disponível em:
KUMAR, Prashant; SAROJ, Devendra P. Water–energy–pollution nexus for growing cities. Urban Climate, v. 10, n. P5, p. 846–853, 1 dez. 2014. Disponível em:
LANDY, M. et al. Bacillomycin: An Antibiotic from Bacillus subtilis Active against Pathogenic Fungi. Experimental Biology and Medicine, v. 67, n. 4, p. 539–541, abr. 1948.
LIN, Long et al. Improving the sustainability of organic waste management practices in the food-energy-water nexus: A comparative review of anaerobic digestion and composting. Renewable and Sustainable Energy Reviews, v. 89, p. 151–167, jun. 2018. Disponível em:
MORITA, Tokio; ASSUMPÇÃO, Rosely Maria Viegas. Manual de Soluções, Reagentes e Solventes. 2. ed. [S.l.]: Edgard Blucher, 2007.
MUKHERJEE, Soumen; DAS, Palashpriya; SEN, Ramkrishna. Rapid quantification of a microbial surfactant by a simple turbidometric method. Journal of Microbiological Methods, v. 76, n. 1, p. 38–42, jan. 2009. Disponível em:
PELIZER, L.L.; PONTIERI, M.H.; MORAES, I.O. Utilização de resíduos agroindustriais em processo biotecnológicos como perspectiva de redução do impacto ambiental. Journal of Technology Management & Innovation, v. 2, p. 118- 127., 2007.
PEREIRA, Jorge F.B. et al. Optimization and characterization of biosurfactant production by Bacillus subtilis isolates towards microbial enhanced oil recovery applications. Fuel, v. 111, p. 259–268, 2013. Disponível em:
PETKOVA, B et al. Foamability of aqueous solutions: Role of surfactant type and concentration. Advances in Colloid and Interface Science, v. 276, p. 102084, fev. 2020. Disponível em:
PRADO, Aline Alves Oliveira Santos et al. Evaluation of a new strategy in the elaboration of culture media to produce surfactin from hemicellulosic corncob liquor. Biotechnology Reports, v. 24, p. e00364, 1 dez. 2019. Disponível em:
RAZA, Zulfiqar Ali; AHMAD, Naseer; KAMAL, Shahid. Multi-response optimization of rhamnolipid production using grey rational analysis in Taguchi method. Biotechnology Reports, v. 3, p. 86–94, 1 set. 2014. Disponível em:
SOUSA, M. et al. Performance of a biosurfactant produced by Bacillus subtilis LAMI005 on the formation of oil / biosurfactant / water emulsion: study of the phase behaviour of emulsified systems. Brazilian Journal of Chemical Engineering, v. 31, n. 3, p. 613–623, set. 2014. Disponível em:
SVENSSON, Erik; GUDMUNDSSON, Magnus; ELIASSON, Ann-Charlotte. Binding of sodium dodecylsulphate to starch polysaccharides quantified by surface tension measurements. Colloids and Surfaces B: Biointerfaces, v. 6, n. 4–5, p. 227–233, 22 maio 1996. Disponível em:
SYED, Shameer; CHINTHALA, Paramageetham. Heavy Metal Detoxification by Different Bacillus Species Isolated from Solar Salterns. Scientifica, v. 2015, p. 1–8, 2015. Disponível em:
TEÓFILO, Reinaldo F.; FERREIRA, Márcia M. C. Quimiometria II: planilhas eletrônicas para cálculos de planejamentos experimentais, um tutorial. Química Nova, v. 29, n. 2, p. 338–350, abr. 2006. Disponível em:
VAN DER GREEF, Jan; SMILDE, Age K. Symbiosis of chemometrics and metabolomics: Past, present, and future. Journal of Chemometrics, v. 19, n. 5–7, p. 376–386, maio 2005. Disponível em:
VECINO, Xanel et al. Sewage Sludge Polycyclic Aromatic Hydrocarbon (PAH) Decontamination Technique Based on the Utilization of a Lipopeptide Biosurfactant Extracted from Corn Steep Liquor. Journal of Agricultural and Food Chemistry, v. 63, n. 32, p. 7143–7150, 2015.
Publicado
2022-03-03
Como Citar
Gomes, W., Martini Rodrigues, B., Andréa Franco Burguim, J., Mendez Soares, D., Etchegaray, A., Kelly Mendes Valente, R., & Borin Nogueira, A. (2022). TENDÊNCIAS DE OTIMIZAÇÃO NA PRODUÇÃO DE LIPOPEPÍDEOS TOTAIS POR Bacillus velezensis 0G REVELARAM UM MÉTODO DE FERMENTAÇÃO SUBMERSO SUSTENTÁVEL USANDO CASCAS DE BATATA DOCE. Revista Tecnológica, 30(1), 107-123. https://doi.org/10.4025/revtecnol.v30i1.55243
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Os autores podem manter os direitos autorais pelo seu trabalho, mas repassam direitos de primeira publicação à revista. A revista poderá usar o trabalho para fins não-comerciais, incluindo direito de enviar o trabalho em bases de dados de Acesso Livre.
