Variabilidade genética entre formae speciales de Fusarium oxysporum e raças 1 e 2 de F. oxysporum f. sp. lycopersici  através de RAPD e sequências de regiões ITS e rDNA

Eiko Eurya Kuramae; Nilton Luiz de Souza

doi:10.4025/actasciagron.v24i0.2409

Genetic variability among formae especiales of Fusarium oxysporum and races 1 and 2 of F. oxysporum F. sp. Lycopersici by RAPD and DNA sequences of ITS and rDNA

Authors

Eiko Eurya Kuramae UNESP Author
Nilton Luiz de Souza UNESP Author

DOI:

https://doi.org/10.4025/actasciagron.v24i0.2409

Keywords:

Fusarium oxysporum, variabilidade genética, ITS

Abstract

Differentiation from formae specials and races of Fusarium oxysporum by morphological and cultural characteristics is difficult and uncertain. Random amplified polymorphic DNA (RAPD) markers and ITS1 and ITS2 (internal transcribed spacer) sequences were used to evaluate genetic variability among four different formae specials of Fusarium oxysporum (F. o. cubense, F. o. lycopersici, F. o. phaseoli and F. o. vasinfectum) and two races (1 and 2) of F. o. lycopersici. Genetic variability up to 50% was observed among the formae specials and 7% between races 1 and 2 of F. o. lycopersici using RAPD molecular markers. Similarity more than 97.2% among formae specials and races 1 and 2 of F. o. lycopersici was observed at ITS1 and ITS2 regions, whereas the 5.8S rDNA gene was 100% homologous among isolates

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Author Biography

Eiko Eurya Kuramae, UNESP

possui graduação pela Universidade Estadual Paulista Júlio de Mesquita Filho (1987) , mestrado em Molecular Biology pela Vrije Universiteit Brussel (1991) , doutorado em Ciencias Biologicas (Genetica) pela Universidade Estadual Paulista Júlio de Mesquita Filho (1995) e pos-doutorado pela Universidade Estadual Paulista Júlio de Mesquita Filho (2000) . Atualmente é PESQUISA E DESENV. da Universidade Estadual Paulista Júlio de Mesquita Filho. Atuando principalmente nos seguintes temas: Colletotrichum Spp., Rapd (Random Amplified Polymorphic Dna), Postbloom Fruit Drop Disease Currículo Lattes

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Published

2008-04-30

Issue

Vol. 24 (2002)

Section

Agronomy

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How to Cite

Genetic variability among formae especiales of Fusarium oxysporum and races 1 and 2 of F. oxysporum F. sp. Lycopersici by RAPD and DNA sequences of ITS and rDNA. (2008). Acta Scientiarum. Agronomy, 24, 1481-1485. https://doi.org/10.4025/actasciagron.v24i0.2409