Viabilidade do grão de pólen de acessos de capim-elefante, milheto - DOI: 10.4025/actascibiolsci.v28i1.1052

Vânia Helena Techio; Lisete Chamma Davide; Cássia Ângela Pedrozo; Antônio Vander Pereira

doi:10.4025/actascibiolsci.v28i1.1052

Pollen viability in elephantgrass, pearl millet and interspecific hybrids - DOI: 10.4025/actascibiolsci.v28i1.1052

Authors

Vânia Helena Techio UNC Author
Lisete Chamma Davide UFLA Author
Cássia Ângela Pedrozo UFLA Author
Antônio Vander Pereira EMBRAPA - MG Author

DOI:

https://doi.org/10.4025/actascibiolsci.v28i1.1052

Keywords:

viabilidade de pólen, Pennisetum, corantes, germinação in vitro

Abstract

The aim of this work was to estimate the pollen viability in elephantgrass, pearl millet and interspecific hybrids accessions through in vitro germination and staining with propionic carmine, acetic orcein and Alexander’s stain. The germination tests were accomplished with fresh pollen grains and stored for 30 days. The elephantgrass and peare millet accessions presented a high pollen viability. Just one of the accessions, when evaluated with Alexander's stain, showed low fertility. For the hybrid, a high rate of functional pollen was observed with the nuclear stain. However, Alexander's stain confirmed the complete sterility of those accessions. Elephantgrass and pearl millet accessions presented a low percentage of viable pollen grain, when induced to germination in culture medium. Complete sterility of the pollen grain was verified in the hybrid, agreeing with the meiotic abnormalities already described in the literature. After storage, the pollen grains of all accessions lost entirely their germinative power

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Author Biography

Vânia Helena Techio, UNC

Possui graduação em Ciências Habilitação Em Biologia pela Fundação Educacional do Alto Uruguai Catarinense (1988), mestrado em Agronomia (Genética e Melhoramento de Plantas) pela Universidade Federal de Lavras (1998) e doutorado em Agronomia (Genética e Melhoramento de Plantas) pela Universidade Federal de Lavras (2002). Atualmente é professor titular da Universidade do Contestado Campus Concórdia. As linhas de pesquisa são citogenética e mutagenicidade Currículo Lattes

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Published

2008-03-11

Issue

Vol. 28 No. 1 (2006)

Section

Botany

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How to Cite

Pollen viability in elephantgrass, pearl millet and interspecific hybrids - DOI: 10.4025/actascibiolsci.v28i1.1052. (2008). Acta Scientiarum. Biological Sciences, 28(1), 7-12. https://doi.org/10.4025/actascibiolsci.v28i1.1052