Effect of different culture medium components on production of alkaloid in callus tissues of Cereus peruvianus (Cactaceae) - DOI: 10.4025/actascibiolsci.v27i1.1355

Abstract

The production of alkaloids from Cereus peruvianus callus cultured in medium supplemented with different tyrosine concentrations, different 2,4-D/kinetin levels, and with NaCl was investigated using long-term callus culture. α- and β-Esterase isozyme patterns were also analyzed to investigate the differential gene expression in callus subcultured with the different tyrosine concentrations. The greatest amounts of alkaloids were obtained from callus maintained in medium containing 200 mg/L of tyrosine. Tyrosine induced a differential gene expression for the synthesis of specific α- and β -esterase isozymes. Two α-carboxylesterases (EC 3.1.1.1) and one α/β-arylesterase (EC 3.1.1.2) were induced in callus, one α/β-acetylesterase (EC 3.1.1.6) and two α-carboxylesterase were detected as more intensely stained while two α/β-carboxylesterases were absent or detected as more weakly stained bands after the addition of tyrosine as precursor