Xylanase production by a wild strain of Aspergillus nidulans - DOI: 10.4025/actascibiolsci.v25i1.2095
DOI:
https://doi.org/10.4025/actascibiolsci.v25i1.2095Keywords:
Aspergillus nidulans, culturas submersas, sabugo de milho, xilanasesAbstract
A wild strain of Aspergillus nidulans isolated from soil produce cellulase-free xylanase activity when developed on submerged cultures using corn cob powder as the main substrate. Maximum xylanase production (220 U/mL) was obtained when the strain was developed in mineral medium supplemented with 3% (w/v) corn cob for 6 days. SDS-PAGE revealed the occurrence of four isoxylanases with molecular weights of 50, 43, 20 and 18 kDa. Crude xylanase was resistant to acetone precipitation with 80% of recovery. The enzyme had optimal activity at pH values between 5.0 and 6.0 and temperature between 50-55oC. The enzyme exhibited high stability under alkaline conditions and temperature up to 55oC. It retained 50% and 23% of its activity when heated for 1 h at 60 and 65oC, respectivelyDownloads
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Published
2008-04-18
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Biology Sciences
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How to Cite
Xylanase production by a wild strain of Aspergillus nidulans - DOI: 10.4025/actascibiolsci.v25i1.2095. (2008). Acta Scientiarum. Biological Sciences, 25(1), 221-225. https://doi.org/10.4025/actascibiolsci.v25i1.2095
