Detecção por SDS-PAGE de um marcador específico no cultivar de mandioca, Fécula Branca, Manihot esculenta Crantz (Euphorbiaceae)

Ãlvaro Júlio Pereira; Sandra Aparecida de Oliveira Collet; Pedro Soares Vidigal Filho; Maria de Fátima Pires da Silva Machado

doi:10.4025/actascibiolsci.v24i0.2365

SDS-PAGE detection of a specific marker in ‘Fécula Branca’ cassava cultivar, Manihot esculenta Crantz (Euphorbiaceae)

Authors

Ãlvaro Júlio Pereira UEM Author
Sandra Aparecida de Oliveira Collet UEM Author
Pedro Soares Vidigal Filho UEM Author
Maria de Fátima Pires da Silva Machado Biologia - UEM Author

DOI:

https://doi.org/10.4025/actascibiolsci.v24i0.2365

Keywords:

cultivares de mandioca, proteínas, SDS-PAGE, patogênese em mandioca

Abstract

Proteins from young unexpanded leaves of seven cassava cultivars Manihot esculenta, Crantz (Euphorbidaceae) were investigated through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The comparison was made through the protein patterns obtained, and their relative amounts determined in plants leaves infected or not with the bacteria Xanthomonas axonopodis pv. manihotis. The electrophoretic protein pattern obtained from the investigated cultivars (“Fibra”, “Fécula Branca”, “Branca de Santa Catarina”, “Verdinha”, “IAC-12”, “IAC-13”, and “IAC-14”) showed a polypeptide subunit present exclusively in the “Fécula Branca” cultivar, with a molecular weight of 93.5 kDa. Also, protein fractions were more intensely stained in young leaves of M. esculenta plants infected with the bacteria. SDS-PAGE did not detect specific markers for pathogenesis, but the 93.5 kDa protein fraction can be used as a molecular marker for the “Fécula Branca” cultivar.

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Author Biography

Maria de Fátima Pires da Silva Machado, Biologia - UEM

Possui graduação em Licenciatura Em Ciências Biológicas pela Universidade de São Paulo (1978) , mestrado em Ciências Biológicas (Biologia Genética) pela Universidade de São Paulo (1982) , doutorado em Ciências Biológicas (Biologia Genética) pela Universidade de São Paulo (1986) e pos-doutorado pelo Instituto de Biologia Celular de Madrid C S I C (1989) . Atualmente é Professor Associado da Universidade Estadual de Maringá, Revisor de periódico da Ciência Rural e Revisor de periódico da Acta Scientiarum (UEM). Tem experiência na área de Genética , com ênfase em Genética Animal. Atuando principalmente nos seguintes temas: G-3-PDH (EC 1.1.1.8), ISOENZIMAS, HIMENOPTEROS. Currículo Lattes

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Published

2008-05-13

Issue

Vol. 24 (2002)

Section

Biology Sciences

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How to Cite

SDS-PAGE detection of a specific marker in ‘Fécula Branca’ cassava cultivar, Manihot esculenta Crantz (Euphorbiaceae). (2008). Acta Scientiarum. Biological Sciences, 24, 615-618. https://doi.org/10.4025/actascibiolsci.v24i0.2365