Eugenol concentrations for deep anesthesia and acute toxicity in piavuçu (Leporinus macrocephalus) juveniles - DOI: 10.4025/actascibiolsci.v29i4.838

Abstract

Three works were undertaken to evaluate the anesthetic capacity of eugenol in “piavuçu” juveniles; the influence of different concentrations on the anaesthetic effect, the acute toxicity (LD 50) and the Time for Lethal Dose (TLD) for the species. Seventy-two fish (1.77 + 0.69 g) were submitted to eight different eugenol concentrations (25; 37.5; 50; 62.5; 75; 100; 125 and 150 mg L^-1); 150 fish were submitted to five concentrations (25; 37.5; 50; 62.5; 75 mg L^-1) for ten minutes; and 150 fish were submitted to 37.5 mg/L for five time intervals (300; 600; 900; 1200; 1500 seconds). Eugenol concentration had strong influence on the anesthetic effect for the species; LD for 600 seconds of exposure were LD01 25.21 mg L^-1, LD50 45.13 mg L^-1 and LD99 65.05 mg L^-1. The TLD were TLD01 322.8 seconds, TLD50 854.9 seconds and TLD99 1386.9 seconds. For fast and safe anesthesia of piavuçu juveniles, 37.5 mg L^-1 of eugenol is recommended.