PROGRAMAÇÃO METABÓLICA NA LACTAÇÃO ASSOCIADA À RESTRIÇÃO CALÓRICA MODERADA INTERFERE NOS PARÂMETROS SANGUÍNEOS E MORFOMETRIA DO COLO DE RATOS ADULTOS

Suellen Cristina Passos; Monica dos Santos Mucio  Loiola; Nayra Thais Delatorre  Branquinho; Maria Raquel Marçal  Natali

doi:10.4025/arqmudi.v28i2.72012

Suellen Cristina Passos UEM https://orcid.org/0009-0005-3291-7432
Monica dos Santos Mucio Loiola https://orcid.org/0000-0003-3431-924X
Nayra Thais Delatorre Branquinho https://orcid.org/0000-0002-8570-5143
Maria Raquel Marçal Natali https://orcid.org/0000-0002-7486-2581

DOI: https://doi.org/10.4025/arqmudi.v28i2.72012

Abstract

The postnatal nutritional environment can contribute significantly to overweight, and in rodents the reduction in offspring numbers during lactation is a classic model of early induction of obesity. The objective of this work was to evaluate the metabolic programming and the effects of moderate caloric restriction (30%), followed or not by free feeding, on the proximal colon of adult male Wistar rats from reduced litters. The experimental groups (n=05) were distributed as follows: control (G9); reduced litter control (G3L); reduced litter and 30% calorie restriction from 60 to 150 days (G3R) and reduced litter followed by 30% calorie restriction from 60 to 90 days with free feeding from 91 to 150 days (G3RL). After 150 days of age, the animals were euthanized to evaluate body weight, nasoanal length, Lee Index, visceral adipose tissue weight, blood parameters, gastrointestinal transit time, morphometry of the proximal colon and quantification of goblet and serotonergic cells in the colonic mucosa. Litter reduction during lactation was effective in inducing obesity in adult rats, and free refeeding of these animals after a period of 30 days of moderate caloric restriction (30%) showed that the metabolic programming during lactation was maintained in adult animals. However, adult rats from reduced litters when subjected to 30% caloric restriction for a period of 90 days achieved improvement in metabolic conditions, reduction in visceral adiposity and gastrointestinal transit time, with readjustment of morphometric parameters of the proximal colon.

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