<b>Development of equipment for decellularization using the perfusion method

Autores

  • Jonathas Haniel Universidade Federal de Minas Gerais http://orcid.org/0000-0002-7234-5513
  • Ana Paula Macedo de Souza Brandão Universidade Federal de Minas Gerais
  • Rosana de Carvalho Cruz Universidade Federal de Minas Gerais
  • Marcos Vale Hospital Nossa Senhora das Dores
  • Betânia Maria Soares Universidade Federal de Minas Gerais
  • Rudolf Huebner Universidade Federal de Minas Gerais

DOI:

https://doi.org/10.4025/actascitechnol.v40i1.35349

Palavras-chave:

decellularization, cardiac bioengineering, biomaterials, perfusion process, heart decellularization

Resumo

This study describes the development of equipment capable of transporting decellularization fluid through an organ by perfusion to create an in vitro acellular scaffold that maintains the three-dimensional architecture of the organ. The equipment was designed to be compatible with several existing decellularization protocols and can be used to modify the flow rate, perfusion pressure, and temperature in each decellularization protocol as well as the technique of cannulation of the organ in a controlled manner. The device was tested with chicken hearts and efficiently accomplished decellularization using the perfusion method. Further amendments can be made to improve the efficiency of the decellularization of diverse organs. It is expected that this equipment will aid in the characterization and improvement of the decellularization process and may have future applications in the process of recellularization of different organs.

 

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Biografia do Autor

Jonathas Haniel, Universidade Federal de Minas Gerais

Departamento de Engenharia Mecânica - DEMEC/ UFMG- Laboratório de Bioengenharia

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Publicado

2018-07-01

Como Citar

Haniel, J., Brandão, A. P. M. de S., Cruz, R. de C., Vale, M., Soares, B. M., & Huebner, R. (2018). <b>Development of equipment for decellularization using the perfusion method. Acta Scientiarum. Technology, 40(1), e35349. https://doi.org/10.4025/actascitechnol.v40i1.35349

Edição

Seção

Biotecnologia

 

0.8
2019CiteScore
 
 
36th percentile
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0.8
2019CiteScore
 
 
36th percentile
Powered by  Scopus

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