<b>Activity and operational stability of the immobilized lipase on zirconium phosphate in the absence and presence of polyethylene glycol</b> - DOI: 10.4025/actascitechnol.v28i2.1173

Authors

  • Adriano Aguiar Mendes EEL - USP
  • Bruno César Moreira Barbosa EEL - USP
  • Cleide Mara Farias Soares UNIT
  • Maria Lucia Caetano Pinto da Silva EEL - USP
  • Heizir Ferreira de Castro EEL - USP

DOI:

https://doi.org/10.4025/actascitechnol.v28i2.1173

Keywords:

lipase, imobilização, fosfato de zircônio, polietilenoglicol

Abstract

In the present work, different immobilization procedures were evaluated: direct addition of the lipase on zirconium phosphate (ZrP), and simultaneous addition of lipase and stabilizing agent (polyethylene glycol, MM-1500) on the same support. The selection of this additive was based on its compatibility with the lipase from Candida rugosa according to data previously obtained using controlled pore silica as support. The methodology of lipase immobilization on ZrP in the presence of PEG-1500 supplied more active immobilized derivatives (310.6 U mg-1) when compared with those obtained without addition of PEG-1500 (162.8 U mg-1), providing high activity recovery in the immobilization step (88.7%). Better performance of the immobilized derivative prepared in the presence of PEG was also verified in organic medium (synthesis of butyl butyrate), in terms of activity and operational stability. These results can be attributed to the enzyme coated by the additive that probably increased the access of the substrate to the active site of the lipase

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Author Biography

Heizir Ferreira de Castro, EEL - USP

Heizir Ferreira de Castro Concluiu o doutorado em Quí­mica Microbiológica - The Victoria University of Manchester (Reino Unido) em 1985. Atualmente é docente/ pesquisador da Escola de Engenharia de Lorena/USP. Publicou 84 artigos em periódicos especializados e 197 trabalhos em anais de eventos. Possui 2 livros publicados. Possui 5 processos ou técnicas registrados e outros 11 itens de produção técnica. participou de 4 eventos no Brasil. Orientou 10 dissertações de mestrado e co-orientou 3, co-orientou 3 teses de doutorado, além de ter orientado 34 trabalhos de iniciação cientifica e 1 trabalho de conclusão de curso nas áreas de engenharia quí­mica e microbiologia. Recebeu 10 prêmios e/ou homenagens. entre 1998 e 2006 Participou de 7 projetos de pesquisa, sendo que coordenou 6 destes. Atualmente coordena 2 projetos de pesquisa. atua na área de engenharia quí­mica, com ênfase em processos bioquí­micos. em suas atividades profissionais interagiu com 118 colaboradores em co-autorias de trabalhos cientí­ficos. Em seu Currí­culo Lattes os termos mais freqí¼entes na contextualização da produção cientifica, tecnológica e artistico-cultural são: lipase, immobilization, esterification, immobilized lipase, técnica sol-gel, fatorial design, enzymatic hydrolysis, biodiesel, monoglicerí­deos Currí­culo Lattes

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Published

2008-03-20

How to Cite

Mendes, A. A., Barbosa, B. C. M., Soares, C. M. F., Silva, M. L. C. P. da, & Castro, H. F. de. (2008). <b>Activity and operational stability of the immobilized lipase on zirconium phosphate in the absence and presence of polyethylene glycol</b> - DOI: 10.4025/actascitechnol.v28i2.1173. Acta Scientiarum. Technology, 28(2), 133–140. https://doi.org/10.4025/actascitechnol.v28i2.1173

Issue

Vol. 28 No. 2 (2006)

Section

Chemical Engineering

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