Antibacterial effect of Raphanus sativus (Radish) root juice on growth performance and caecal bacterial load of broiler chickens

Oluwagbemiga Olanrewaju  Adeleye; Bukola Christiana  Majekodunmi; Munirat Olaide  Abatan; Tolulope Mobolaji  Obuotor; Amos Oyebisi Kolawole Kolawole

doi:10.4025/actascibiolsci.v46i1.68630

Autores

Oluwagbemiga Olanrewaju Adeleye Federal University of Agriculture Autor
Bukola Christiana Majekodunmi Federal University of Agriculture Autor
Munirat Olaide Abatan Federal University of Agriculture Autor
Tolulope Mobolaji Obuotor Federal University of Agriculture Autor
Amos Oyebisi Kolawole Kolawole Federal University of Agriculture Autor https://orcid.org/0000-0002-3213-1385

DOI:

https://doi.org/10.4025/actascibiolsci.v46i1.68630

Palavras-chave:

Radish; broiler; growth performance; antibacterial activity; caecum bacterial load

Resumo

Majority of poultry producers have been concerned about the microbial colonization of chicken guts, which usually result in competition for nutrient and poor health. As a way to combat this, antibiotics are used, however, this has led to antibiotic resistance and the presence of antibiotic residues in poultry products. Therefore, the purpose of this study is to ascertain the effect of radish root juice on the microbiota in the caecum of broiler chickens while also monitoring their growth response. This study was a 2 by 3 factorial layout. There were six treatment groups of forty day-old broiler chicks each, consisting of two dosages of radish juice (10 mL L^-1 or 15mL L^-1 of the birds’ drinking water) and three frequencies of administration (0, 2 and 3 times weekly). The zero frequencies (no juice) were the control groups given Enrofloxacin as antibiotic (1 mL in 4 L of drinking). At the starter and finisher phase, birds were given commercial diets. The growth performance was evaluated at both phases, while the microbial load for each group was determined at the finisher phase. The contents of the birds' caeca were taken for microbial analysis at 42^nd day of the experiment. Frequency and nature of microorganism were obtained. Microbial count of broilers administered radish juice ranged from 7.84 x 10⁷ – 1.75 x 10⁷ cfu mL^-1 while those administered antibiotics had 8.75 x 10⁷ – 8.56 x10⁷ cfu mL^-1. Microorganisms isolated include: Klebsiella Pneumoniae (27%), Morganella morganii (24%), Bacillus subtilis (22%), Providencia rettgerii (5%), Staphylococcus saprophyticus (5%), Staphylococcus aureus (4%), Citrobacter fruendii (4%), Proteus mirabilis (2%), Micrococcus luteus (2%), Escherichia coli (2%), Providencia stuartii (2%). There was no statistical difference (P>0.05) among all the growth parameters in both the radish groups and the antibiotic groups, at the starter and finisher phase. This study suggests radish root juice as an effective antimicrobial agent in reducing microbial load of the gut of broilers which consequently can lead to increased productivity and feed – to – meat conversion ratio.

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