INTESTINAL AND HEPATIC MORPHOLOGICAL EVALUATION IN OBESE RATS: EFFECTS OF Moringa oleifera LEAF EXTRACT
Abstract
Obesity is a serious public health problem, associated with the development of several comorbidities. Moringa oleifera is an herbal medicine known for its chemical properties that can contribute to the control of obesity by reducing the lipid profile and liver inflammation. The objective of this work was to evaluate the effects of using an aqueous solution of M. oleifera (200mg/kg of body mass) for 60 days, as a therapeutic alternative for obesity caused by the ingestion of a diet rich in simple carbohydrates (RSC) through of liver and jejunum morphological analysis of Wistar rats. The following groups were formed: water control (CC): feeding with a standard diet and gastric gavage with filtered water; Moringa control (CM): feeding with a standard diet and Moringa gastric gavage; obese water (OB): feeding with RSC diet and gastric gavage with filtered water; Moringa obese (OM): feeding with RSC diet and gastric gavage with Moringa. At 150 days, after euthanasia, liver and jejunum samples were fixed for histological processing and staining with HE for morphological and morphometric analyses, and PAS histochemical reaction for quantification of goblet cells and tissue glycogen. Oral administration of M. oleifera in obese rats reduced body mass and, in the liver, reduced the area of hepatocytes with an increase in cell number and an increase in the percentage of tissue glycogen. In the jejunum, M. oleifera reduced the thickness of the intestinal tunics and wall and the goblet cell index, which was increased in obese groups, suggesting an improvement in nutrient absorption and intestinal defense.
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